| Objective To investigate whether higenamine and ginsenoside Rg1 in Shenfu decoction extracts regulate mitochondrial function in rats with heart failure by regulating heat shock protein 70 (HSP70) through microRNA-1 (miRNA-1). Methods 100 Wistar rats were selected, 20 of them were routinely raised, and the remaining 80 rats were established with adriamycin-induced heart failure rat model. The HG group intervened with fenugreek, the Rg1 group intervened with 5 mg/kg ginsenoside Rg1, and the HG/Rg1 group intervened with higenamine combined with ginsenoside Rg1. After 4 weeks, the cardiac function, B-type natriuretic peptide (BNP), myocardial histopathology, HSP70 in myocardial tissue, mitochondrial function indexes adenosine triphosphate (ATP), reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were observed in each group. , mitochondrial ultrastructure and myocardial tissue miRNA-1 expression. Results Compared with the normal group, the levels of LVESD, LVEDD, LVESV, LVEDV, LVAWDd, LVAWDs, LVPWDd, LVPWDs, BNP, ROS, miRNA-1 expression in model group, HG group, Rg1 group, HG/Rg1 group increased, and the levels of EF, FS, ATP, MMP, HSP70 decreased (P<0.05); Compared with the model group, the levels of LVESD, LVEDD, LVESV, LVEDV, LVAWDd, LVAWDs, LVPWDd, LVPWDs, BNP, ROS and miRNA-1 in HG group, Rg1 group and HG/Rg1 group decreased, and the levels of EF, FS, ATP, MMP and HSP70 increased (P<0.05); Compared with HG group and Rg1 group, the levels of LVESD, LVEDD, LVESV, LVEDV, LVAWDd, LVAWDs, LVPWDd, LVPWDs, BNP, ROS, miRNA-1 expression in HG/Rg1 group decreased, and the levels of EF, FS, ATP, MMP, HSP70 increased (P<0.05); There was no significant difference between HG group and Rg1 group (P>0.05). Conclusion The combination of the extract of Shenfu decoction, noraconitine and ginsenoside Rg1 may protect the myocardial cells by inhibiting miRNA-1 to up regulate HSP70 and maintaining the structure and function of mitochondria in rats with heart failure. |
