| 余文胜,黄飞,郑妮.桑枝多糖基于YAP/ TAZ信号通路调控db/db小鼠肾纤维化作用的研究[J].浙江中西医结合杂志,2021,31(7): |
| 桑枝多糖基于YAP/ TAZ信号通路调控db/db小鼠肾纤维化作用的研究 |
| Study of Mulberry Branch Polysaccharide in Regulating Renal Fibrosis of db/db Mice Based on YAP/TAZ Signal Pathway |
| 投稿时间：2021-03-10 修订日期：2021-06-09 |
| DOI： |
| 中文关键词: 桑枝多糖 肾纤维化 YAP/ TAZ db/db小鼠 |
| 英文关键词:Mulberry Branch Polysaccharide Renal fibrosis YAP/TAZ db/db mice |
| 基金项目:国家自然科学青年基金(81500633) |
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| 摘要点击次数: 331 |
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| 中文摘要: |
| 目的：研究桑枝多糖基于YAP/ TAZ信号通路调控db/db小鼠肾纤维化作用的影响。方法： |
| 英文摘要: |
| Objective: To investigate the effect of Mulberry Branch Polysaccharide in regulating renal fibrosis of db/db mice based on YAP/TAZ signal pathway. Methods: Twenty four weeks’ old spontaneous db/db type 2 diabetic mice were randomly divided into model group, valsartan (20 mg/kg) group, Mulberry Branch Polysaccharide (600, 1200 mg/kg) groups with 10 mice per group. The mice were fed with high fat diet, and were intragastrically administered with drugs once a day for 60 days. The mice in normal group were fed with standard rodent food and given same volume of saline for 60 days. The urine samples were collected after 60 days’ administration. The urine volume and 24 h urine albumin (UP) were measured. And the serum creatinine (Scr) and urea Nitrogen (BUN) were determined by automatic biochemical analyzer. The fasting blood glucose (FBG) was detected after last administration with 12 h fast. Additionally, the renal samples were collected for HE staining. The renal levels of fibrosis indicators TGF-β1, fibronectin (FN), connective tissue growth factor (CTGF), collagen type I (Collagen I), inflammatory factors tumor necrosis factor (TNF-α), interleukin-6 (IL-6) and Chemokine Monocyte Chemotactic Protein-1 (MCP-1) were measured by ELISA. The expressions of Yes related protein (YAP) and TAZ in renal tissues were analyzed. Results: Compared with model group, the Scr (0.39±0.09、0.32±0.07 mg/dl) and BUN (25.75±4.75、20.62±3.68 mg/dl) levels were significantly decreased in mice treated with Mulberry Branch Polysaccharide (P<0.05). The TGF-β1 (3.76±0.52、2.53±0.38 ng/mL), FN (5.29±1.13、4.47±1.02 ng/mL), CTGF (14.52±1.22、11.24±0.87 ng/mL), CollagenⅠ (10.93±0.85、8.14±0.76 ng/mL), TNF-α (87.25±7.81、78.66±6.36 ng/L), IL-6 (34.72±3.35、21.16±2.51 ng/L) and MCP-1 (0.38±0.04、0.29±0.06 ng/L) (P<0.05). The expressions of YAP and TAZ were markedly down regulated (P<0.05). Conclusion: The protective effect of Mulberry Branch Polysaccharide on renal fibrosis of db/db mice may be associated with regulating YAP/TAZ signal pathway, reducing the formation of fibrotic factors in kidney tissue and the content of inflammatory factors, thereby playing a protective role. |
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