| 郑凌歆,王金丹,何超翔,郑兰芝.麦冬多糖抑制TGF-β1诱导的人胚胎肺成纤维细胞表型的转化[J].浙江中西医结合杂志,2021,31(7): |
| 麦冬多糖抑制TGF-β1诱导的人胚胎肺成纤维细胞表型的转化 |
| Ophiopogon japonicus polysaccharide inhibits TGF-β1 induced phenotypic transformation of human embryonic lung fibroblasts |
| 投稿时间：2021-01-31 修订日期：2021-04-20 |
| DOI： |
| 中文关键词: TGF-β1 麦冬多糖 人胚胎肺成纤维细胞 α-SMA |
| 英文关键词:TGF- β 1 Ophiopogon japonicus polysaccharides human embryonic lung fibroblasts α-SMA |
| 基金项目:浙江中医药科技计划（编号：2018ZB041） |
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| 中文摘要: |
| 研究麦冬多糖对转化生长因子-β1(TGF-β1)诱导的人胚胎肺成纤维细胞表型转化的影响,并初步探讨其分子机制。方法 采用TGF-β1诱导人胚胎肺成纤维细胞(HEL)转化,同时给予麦冬多糖进行干预；采用CCK-8检测细胞的增殖率,电镜观察细胞的超微结构,Western blot检测α-平滑肌肌动蛋白(α-SMA)、Ⅰ 型胶原蛋白(COL I)、Smad2蛋白和p-Smad2蛋白的表达,实时荧光定量RT-PCR检测α-SMA mRNA和COL Ⅰ mRNA的表达。结果 25、50、100 μg.ml-1浓度的麦冬多糖对HEL细胞均无明显毒性,并能抑制TGF-β1诱导所致的成纤维细胞增殖(P＜0.05)；与对照组相比,麦冬多糖干预组细胞表面微绒毛减少、微丝变短,胞浆内线粒体数目下降,粗面内质网减少； 同时下调α-SMA、COL I、Smad2、p-Smad2蛋白的表达量和α-SMA、COL I的mRNA表达水平(P＜0.05)。结论 麦冬多糖可能通过干预TGF-β/Smads信号通路,在一定程度上抑制成纤维细胞向肌成纤维细胞的转化。 |
| 英文摘要: |
| Objective To investigate the effect of Ophiopogon japonicus polysaccharides on phenotypic transformation of human embryonic lung fibroblasts induced by transforming growth factor-β1 (TGF-β1), and to explore its molecular mechanism. Methods Transformation of human embryonic lung fibroblasts(HEL) was induced by TGF-β1 and interfered with different concentrations of Ophiopogon japonicus polysaccharides. The proliferation rate of cells was detected by CCK-8, the expression of α-smooth muscle actin (α-SMA), type I collagen (COL I), Smad2 protein and p-Smad2 protein was detected by electron microscope, and the expression of α-SMA mRNA and COL Ⅰ mRNA was detected by real-time fluorescence quantitative RT-PCR. Results Different concentrations of Ophiopogon japonicus polysaccharides had no obvious toxic effect on cells, and could inhibit the proliferation of fibroblasts induced by TGF-β1 (P＜0.05). Compared with the control group, the surface microvilli, microfilaments, mitochondria and rough endoplasmic reticulum decreased in Ophiopogon japonicus polysaccharides group. Down-regulated the expression of α-SMA, COL I, Smad2, p-Smad2 protein and the mRNA expression level of α-SMA and COL I(P＜0.05). Conclusion Ophiopogon japonicus polysaccharides may inhibit the transformation of fibroblasts to myofibroblasts to some extent by interfering with TGF-β/Smads signal pathway. |
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