| 高聚伟,徐凯,冉冉.基于网络药理学探讨澳洲茄碱抗肿瘤机制[J].浙江中西医结合杂志,2021,31(7): |
| 基于网络药理学探讨澳洲茄碱抗肿瘤机制 |
| Anticancer Mechanism Research of Solasonine Via Network Pharmacological Technology |
| 投稿时间：2021-01-15 修订日期：2021-03-11 |
| DOI： |
| 中文关键词: 网络药理学 澳洲茄碱 抗肿瘤 作用靶点 作用机制 |
| 英文关键词:Network Pharmacology Solasonine Anticancer Target of action Mechanism |
| 基金项目:浙江省医药卫生科技计划项目(2017KY519) |
|
| 摘要点击次数: 471 |
| 全文下载次数: 2 |
| 中文摘要: |
| 目的:基于网络药理学，构建澳洲茄碱抗肿瘤的“靶点-通路”网络，探讨澳洲茄碱抗肿瘤的作用机制。方法:通过TCMSP及Pharm Mapper收集澳洲茄碱潜在靶点，OMIM及Genecards数据库收集肿瘤疾病靶点，将澳洲茄碱潜在靶点与肿瘤疾病靶点交集，获得澳洲茄碱-肿瘤共同靶点。运用String数据库结合Cytoscape绘制澳洲茄碱-肿瘤共同靶点的蛋白相互作用网络图(PPI)，筛选核心靶点。最后，基于R3.6.0软件用Bioconductor生物信息软件包，对澳洲茄碱-肿瘤共同靶点进行进行GO功能与KEGG通路富集分析。结果:澳洲茄碱-肿瘤共同靶点79个，核心基因3个：IGF1R、MAP2K1、CHEK1。通路分析显示澳洲茄碱参与多条肿瘤相关通路的调控，主要涉及PI3K-Akt信号通路、MAPK信号通路、Ras信号通路等经典肿瘤信号通路。结论:澳洲茄碱调控多个靶点，同一个靶点参与多个生物学过程和信号通路，澳洲茄碱可能通过多靶点、多通路发挥抗肿瘤作用。 |
| 英文摘要: |
| Abstract: Objective: To construct the “target-pathway” network of Solasonine based on network pharmacology, and explore the anticancer mechanism of Solasonine.
Methods: First, the potential targets of Solasonine were collected through TCMSP and Pharm Mapper. Then, the cancer targets were searched through OMIM and Genecards database. The common targets of Solasonine-cancer were obtained by the intersection of Solasonine targets and cancer targets. Next, based on the String platform, the protein-protein interaction (PPI) network model of the potential anticancer targets was constructed using the Cytoscape software. Finally, gene ontology function and KEGG pathway enrichment analysis of Solasonine-cancer common targets were performed using Bioconductor software package based on R3.6.0 software.
Results: There were 79 common targets of Solasonine-cancer and 3 core genes (IGF1R, MAP2K1 and CHEK1). Pathway analysis showed that Solasonine was involved in the regulation of multiple cancer-related pathways, such as PI3K-Akt signaling pathway, MAPK signaling pathway, Ras signaling pathway, ErbB signaling pathway, and VEGF signaling pathway.
Conclusion: Solanine regulated multiple targets, and the same target participated in multiple biological processes and signaling pathways, suggesting that Solanine exerted anticancer effect through multi-targets and multi-pathways. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
