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冯淑炯.桃叶珊瑚苷对人结肠癌HT-29细胞恶性生物学行为及SCAP/SREBP-1通路的影响[J].浙江中西医结合杂志,2021,31(6):
桃叶珊瑚苷对人结肠癌HT-29细胞恶性生物学行为及SCAP/SREBP-1通路的影响
The effect of aucubin on the malignant biological behavior of human colon cancer HT-29 cells and SCAP/SREBP-1 pathway
投稿时间:2021-01-11  修订日期:2021-01-22
DOI:
中文关键词:  桃叶珊瑚苷  结肠癌  恶性生物学  SCAP/SREBP-1通路
英文关键词:Aucubin  Colon cancer  Malignant biology  SCAP/SREBP-1 pathway
基金项目:
作者单位E-mail
冯淑炯 杭州市老年病医院消化内科 fengjiong29@163.com 
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中文摘要:
      目的 探讨桃叶珊瑚苷对人结肠癌HT-29细胞恶性生物学行为及SREBP裂解激活蛋白(SCAP)/固醇调节元件结合蛋白-1(SREBP-1)通路的影响。方法 设结肠癌HT-29细胞组、5-氟尿嘧啶组(浓度为30μmol/L的5-氟尿嘧啶处理)、桃叶珊瑚苷低剂量组(浓度为50μmol/L桃叶珊瑚苷处理)、桃叶珊瑚苷高剂量组(浓度为100μmol/L桃叶珊瑚苷处理),各组每孔设6个平行样,培养72h;培养结束后,采用四唑盐(MTT)法测定细胞增殖,流式细胞仪测定细胞凋亡水平,Transwell腔室系统测定细胞侵袭迁移能力,实时荧光定量PCR及蛋白免疫印迹法测定细胞SCAP、SREBP-1 mRNA与蛋白水平。结果 与结肠癌HT-29细胞组比较,5-氟尿嘧啶组、桃叶珊瑚苷低、高剂量组OD值、存活率、穿膜数、细胞SCAP、SREBP-1 mRNA和蛋白水平降低,凋亡率升高(P<0.05);与5-氟尿嘧啶组比较,桃叶珊瑚苷低、高剂量组OD值、存活率、穿膜数、细胞SCAP、SREBP-1 mRNA和蛋白水平升高,凋亡率降低(P<0.05);与桃叶珊瑚苷低剂量组比较,桃叶珊瑚苷高剂量组OD值、存活率、穿膜数、细胞SCAP、SREBP-1 mRNA和蛋白水平降低,凋亡率升高(P<0.05)。结论 桃叶珊瑚苷能明显抑制人结肠癌HT-29细胞增殖、侵袭,促进其凋亡;其机制可能与桃叶珊瑚苷抑制SCAP/SREBP-1通路的激活,进而抑制脂肪的合成有关。
英文摘要:
      Objective: To investigate the effects of aucubin on malignant biological behavior and SREBP cleavage activating protein (SCAP) / sterol regulatory element binding protein-1 (SREBP-1) pathway in human colon cancer HT-29 cells. Methods: The colon cancer HT-29 cell group, 5-fluorouracil group (30μmol/L 5-fluorouracil treatment), aucubin low dose group (50μmol/L aucubin treatment), aucubin high dose group(100μmol/L aucubin treatment) were set up, and each group set up 6 parallel samples per well and cultured for 72 hours. After the end of the culture, the cell proliferation was measured by tetrazolium salt(MTT) method, the apoptosis level measured by flow cytometry, Transwell chamber system was used to measure cell invasion and migration, real-time PCR and Western blotting were used to determine the mRNA and protein levels of SCAP and SREBP-1 in cells. Results: Compared with colon cancer HT-29 cell group, the OD value, survival rate, number of transmembrane, cell SCAP, SREBP-1 mRNA and protein levels in the 5-fluorouracil group, aucubin low and high dose group were decreased, the apoptosis rate increased(P<0.05). Compared with the 5-fluorouracil group, the OD value, survival rate, number of transmembrane, cell SCAP, SREBP-1 mRNA and protein levels in the aucubin low dose and high dose groups were increased, the apoptosis rate decreased (P<0.05). Compared with the aucubin low dose group, the OD value, survival rate, number of transmembrane, cell SCAP, SREBP-1 mRNA and protein levels in the aucubin high dose group were decreased, the apoptosis rate increased(P<0.05). Conclusion: Aucubin can obviously inhibit the proliferation and invasion of human colon cancer HT-29 cells, and promote apoptosis. Its mechanism may be related to the inhibition of the activation of SCAP/SREBP-1 pathway by aucubin, thereby inhibiting the synthesis of fat.
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