| 张丹娜,叶丽红,刘喜德.润燥活血法对干燥综合征小鼠血清及颌下腺M3R表达的影响[J].浙江中西医结合杂志,2021,31(7): |
| 润燥活血法对干燥综合征小鼠血清及颌下腺M3R表达的影响 |
| Effect of Runzao Huoxue Method on Expression of M3R in blood and submandibular gland of SS Mouse |
| 投稿时间：2020-12-01 修订日期：2021-06-01 |
| DOI： |
| 中文关键词: 润燥活血法 干燥综合征 毒蕈碱样乙酰胆碱3型 血清 颌下腺 免疫组化 |
| 英文关键词:Runzao Huoxue Method Sjogren’s Syndrome muscarinic acethyleholine receptor 3 peripheral blood submandibular gland immunohistochemical |
| 基金项目:浙江省中西医结合医院院内课题(hhyn201904), |
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| 中文摘要: |
| 目的 通过观察干燥综合征(sjogren syndrome，SS)小鼠血清及颌下腺毒蕈碱样乙酰胆碱3型(M3R)的表达，探讨润燥活血法对其的影响及作用机理。方法 选用BALB/c小鼠47只，随机分为正常对照组（正常组，n=10）和造模组（n=37）。取5只BALB/c小鼠处死取颌下腺制备抗原进行造模。造模组采用背部皮内5个部位多点注射抗原的方法进行免疫，建立SS模型。在造模后第5周随机选取2只造模小鼠处死，通过病理形态学观察判定造模成功。造模成功后随机分为模型组（n=10）、中药组（n=10）和HCQ组（n=10）。分别灌胃给药：中药组按11.4g/kg/d剂量灌胃中药解毒通络生津汤，模型组灌胃等量生理盐水，HCQ组小鼠按0.06g/kg/d剂量灌胃HCQ混悬液（HCQ与生理盐水混合液），均每日1次，正常组正常饲养。同时测定小鼠唾液量、饮水量。连续用药30天后处死，取各组小鼠颌下腺、脾及血清。采用苏木素-伊红（HE）染色评价颌下腺淋巴细胞浸润情况，采用ELISA法测定标本血清M3R水平，免疫组化法检测颌下腺M3R表达水平。结果 与正常组比较，模型组血清、颌下腺M3R表达增加，颌下腺淋巴细胞浸润明显[（12.23±10.10，2.23±0.11，2.42±0.22）比（2.72±1.21，1.21±0.11，0.18±0.05），P＜0.05、P＜0.01、P＜0.01]；与模型组比较，中药组、HCQ组血清、颌下腺M3R表达减少，颌下腺淋巴细胞浸润减轻[（3.30±6.25，1.62±0.40，1.21±0.33），（3.17±0.87，1.53±0.06，1.08±0.09）比（12.23±10.10，2.23±0.11，2.42±0.22），均P＜0.05]；中药组和HCQ组之间比较无差异[（3.30±6.25，1.62±0.40，1.21±0.33）比（3.17±0.87，1.53±0.06，1.08±0.09），均P＞0.05]。与治疗前比较，中药组小鼠唾液量增加[（25.53±4.27mg）比（22.17±1.50mg），P＜0.05]。结论 润燥活血法可能通过下调M3R表达，从而抑制颌下腺淋巴细胞浸润，改善腺体分泌能力，阻止SS进展。 |
| 英文摘要: |
| Abstract: Objective:To study the therapeutic effect and mechanism of Runzao Huoxue Method on expression of M3R in of M3R in blood and submandibular gland of SS Mouse.Methods:Forty-seven BALB/c mouse were randomly divided into making model group(n=37) and normal control group(n=10).Submandibular gland of 5 BALB/c mice were made into antigen to molding. Mouse of making model group was induced by intradermal injection of antigen in the backs in 5 sites to establish SS model. At the fifth week after modeling, 2 mice were randomly selected and killed, and the pathological morphology observation was used to determine the success of modeling.The succeeded SS mouse were randomly divided into model control group(n=10),TCM group(n=10) and HCQ group(n=10). Mouse of TCM group were given Jiedu Tongluo Shengjin Decoction by stomach rearing according to 11.4g/kg·d.Mouse of model control group were given equivalent Physiological saline. Mouse of HCQ group were given HCQ liquor according to 0.06g/kg·d in the same way. Normal feeding of normal group.After treatment 30 days, spleen、submandibular gland and peripheral blood were collected. Using HE staining method to examine run situation of lymphocyte in submandibular gland of mouse, using ELISA method to determine standard of M3R in peripheral blood of mouse,and using immunohistochemical to determine expression of M3R in submandibular gland of mouse.Results: Compared with normal group, the expression of m3r in serum and submandibular gland of model group was increased, and lymphocyte infiltration of submandibular gland was obvious [（12.23±10.10，2.23±0.11，2.42±0.22）比（2.72±1.21，1.21±0.11，0.18±0.05），P＜0.05、P＜0.01、P＜0.01]. Compared with the model group, the expression of m3r in serum and submandibular gland was decreased in TCM group and HCQ group[（3.30±6.25，1.62±0.40，1.21±0.33），（3.17±0.87，1.53±0.06，1.08±0.09）比（12.23±10.10，2.23±0.11，2.42±0.22），均P＜0.05]. There was no difference between the two groups[（3.30±6.25，1.62±0.40，1.21±0.33）比（3.17±0.87，1.53±0.06，1.08±0.09），均P＞0.05]. Compared with that before treatment, the amount of saliva in the traditional Chinese medicine group increased[（25.53±4.27mg）比（22.17±1.50mg），P＜0.05].Conclusion:Traditional Chinese medicine of dryness toxin and dredging collaterals method may by reducing the M3R expression, thus inhibiting the run situation of lymphocyte of SS mouse. |
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