| 胡爱荣.桑葚对大鼠非酒精性脂肪性肝病脂质代谢的影响[J].浙江中西医结合杂志,2021,31(5): |
| 桑葚对大鼠非酒精性脂肪性肝病脂质代谢的影响 |
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| 投稿时间：2020-11-02 修订日期：2021-04-11 |
| DOI： |
| 中文关键词: 桑葚 非酒精性脂肪性肝病 大鼠 脂质代谢 |
| 英文关键词:Mulberry extract Nonalcoholic fatty liver disease Rats Lipid metabolism |
| 基金项目:浙江省中医药科技计划项目(2018ZB118)；宁波市消化系统肿瘤临床医学研究中心(2019A21003) |
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| 中文摘要: |
| 目的 探讨桑葚对高脂饮食诱导的大鼠非酒精性脂肪性肝病（NAFLD）脂质代谢的影响。方法 清洁级SD雄性大鼠按随机数字表法随机分为正常组10只（正常饲料）和造模组50只（高脂饲料），持续8周。于第9周起，再将造模组大鼠按随机数字表法随机分为模型组、桑葚低、中、高剂量组、异甘草酸镁对照组，每组各10只。干预组灌胃给予对应药物，正常组与模型组给予等量生理盐水，持续6周。赖氏法测定大鼠血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、酶法测定血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）及高密度脂蛋白胆固醇（HDL-C）的变化，观察肝脏大体观，计算肝脏指数，油红O染色及HE染色观察肝组织脂滴分布状况及病理学变化。结果 与正常组比较：模型组的肝脏大体色泽较为苍白，肝指数下降[（2.86±0.10）比（3.79±0.55），P <0.01]，肝脏油红O染色强阳性，脂肪沉积明显，肝脂肪变显著，血清ALT、AST、TG、TC及LDL-C升高，HDL-C下降[（38.70±7.87）U/L比（72.50±6.20）U/L、（93.60±16.61）U/L比（267.00±40.70）U/L、（0.99±0.09）mmol/L比（1.52±0.24）mmol/L、（1.11±0.07）mmol/L比（1.52±0.14）mmol/L、（0.41±0.07）mmol/L比（0.57±0.04）mmol/L、（0.43±0.03）mmol/L比（0.38±0.05）mmol/L，P均<0.05]。与模型组比较：各干预组肝脏大体色泽的红润度均有一定改善，以桑葚高剂量组的改善最为明显。肝脏指数均有不同程度提高，桑葚中、高剂量组及异甘草酸镁对照组的改善显著[（3.12±0.14）、（3.22±0.10）及（3.16±0.22）比（2.86±0.10），P均<0.01]。肝组织油红O染色强度随桑葚剂量的增加而逐渐减轻，桑葚高剂量组及异甘草酸镁对照组的肝脏脂肪沉积改善显著；肝脂肪变均不同程度改善。桑葚各剂量组血清ALT、AST均下降，呈剂量依赖性[（54.80±5.16）U/L、（53.00±8.65）U/L及（43.30±2.36）U/L比（72.50±6.20）U/L，（135.40±26.68）U/L、（124.60±35.29）U/L及（93.90±8.91）U/L比（267.00±40.70）U/L，P均< 0.01]；TG、LDL-C均下降，呈剂量依赖性[（1.05±0.22）mmol/L、（0.95±0.17）mmol/L及（0.79±0.03）mmol/L比（1.52±0.24）mmol/L，（0.48±0.05）mmol/L、（0.47±0.05）mmol/L及（0.39±0.02）mmol/L比（0.57±0.04）mmol/L，P均< 0.01]；桑葚高剂量组血清TC降低[（1.21±0.08）mmol/L比（1.52±0.14）mmol/L，P < 0.01]，低、中剂量组HDL-C升高[（0.46±0.05）mmol/L及（0.43±0.04）mmol/L比（0.38±0.05）mmol/L，P<0.05、<0.01]。结论 桑葚对高脂饮食诱导NAFLD大鼠的脂质代谢具有良好的改善作用。 |
| 英文摘要: |
| Objective To explore the effects of mulberry on lipid metabolism in rats with high-fat diet induced non-alcoholic fatty liver disease (NAFLD). Methods Sixty male Sprague-Dawley rats were randomly divided into normal group (10 rats, normal feed) and model group (50 rats, high-fat feed) according to the random number table method for 8 weeks. From the 9th week, the model rats were randomly divided into model group, intervention groups (mulberry low dosage, moderate dosage, and high dosage), and positive control group (magnesium isoglycyrrhizinate) according to the random number table method, 10 rats per group. The intervention groups were given the corresponding drugs by gavage, and the normal group and the model group were given the same amount of normal saline for 6 weeks. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), and high density lipoprotein cholesterol (HDL-C) were determined by Reit′s method and enzymatic determination, respectively. The general view of the liver was recorded, the liver indexes were calculated, and the oil red O staining and the HE staining were used to observe the distribution of lipid droplets and the pathologic changes of liver tissue. Results Compared with the normal group, The liver of the model group was pale in general, the liver indexes decreased [(2.86±0.10) vs (3.79±0.55), P <0.01], the oil red O staining was strong, fat deposition was obvious, liver steatosis became significant, serum ALT, AST, TC, TG and LDL-C levels increased, and HDL-C decreased [(38.70±7.87) U/L vs (72.50±6.20) U/L, (93.60±16.61) U/L vs (267.00±40.70) U/L, (0.99±0.09) mmol/L vs (1.52±0.24) mmol/L, (1.11±0.07) mmol/L vs (1.52±0.14) mmol/L, (0.41±0.07) mmol/L vs (0.57±0.04) mmol/L, (0.43±0.03) mmol/L vs (0.38±0.05) mmol/L, P <0.05]. Compared with the model group, the ruddy degree of liver color in each intervention group was improved to some extent, especially in the high dosage group. The liver index of each intervention group was higher than that of the model group, and the improvement of moderate dosage, high dosage group and magnesium isoglycyrrhizate group was significant [(3.12±0.14), (3.22±0.10) and (3.16±0.22) vs (2.86±0.10), P <0.01]. The staining intensity of oil red O and liver steatosis gradually decreased with the increase of the dosage of mulberry extract, and the deposition of liver lipid in high dosage group and magnesium isoglycyrrhizate group improved significantly. The serum ALT, AST, TG, LDL-C levels group decreased in a dose-dependent manner[(54.80±5.16) U/L, (53.00±8.65) U/L and (43.30±2.36) U/L vs( 72.50±6.20) U/L, (135.40±26.68) U/L, (124.60±35.29) U/L and (93.90±8.91) U/L vs (267.00±40.70) U/L，(1.05±0.22) mmol/L, (0.95±0.17) mmol/L and (0.79±0.03) mmol/L vs (1.52±0.24) mmol/L, (0.48±0.05) mmol/L, (0.47±0.05) mmol/L and (0.39±0.02) mmol/L vs (0.57±0.04) mmol/L, respectively, P < 0.01], the serum TC of high dosage group decreased [(1.21±0.08) mmol/L vs (1.52±0.14) mmol/L, P < 0.01], and the serum HDL-C of low and moderate dosage groups increased [(0.46±0.05) mmol/L and (0.43±0.04) mmol/L vs (0.38±0.05) mmol/L, P<0.05 and <0.01]. Conclusion Mulberry extract has a good effect on improving lipid metabolism in rats with high-fat diet induced NAFLD. |
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