浙江中西医结合杂志

梁伟海,陈志宁,林国伟,刘晓俊.天麻、法半夏对ox-LDL诱导的HUVECs损伤的影响[J].浙江中西医结合杂志,2021,31(5):

天麻、法半夏对ox-LDL诱导的HUVECs损伤的影响

The effects of Gastrodia elata and Rhizoma pinelliae preparatum on ox-LDL-induced injury of HUVECs

投稿时间：2020-09-08 修订日期：2021-04-09

DOI：

中文关键词: 天麻法半夏人脐静脉内皮细胞细胞损伤 VEGF VEGFR-2

英文关键词:Gastrodia elata, Rhizoma pinelliae preparatum, HUVECs, cell injury, VEGF, VEGFR-2

基金项目:广东省省级科技计划项目(2017A020213027)；刘晓俊广州市名中医传承工作室建设项目(ky01050002)

作者	单位	E-mail
梁伟海	广州市第一人民医院（华南理工大学第二附属医院）	lwhfimmu@163.com
陈志宁	广州市第一人民医院（华南理工大学第二附属医院）
林国伟	广州市第一人民医院（华南理工大学第二附属医院）
刘晓俊^*	广州市第一人民医院（华南理工大学第二附属医院）	alxj@163.com

摘要点击次数: 476

全文下载次数: 2

中文摘要:

摘要目的：探讨天麻、法半夏对氧化型低密度脂蛋白（ox-LDL）诱导的人脐静脉内皮细胞（HUVECs）损伤的影响。方法：体外培养HUVECs，随机分为对照组、模型组、天麻组（5 mg/mL）、法半夏组（10mg/mL），流式细胞仪检测HUVECs细胞凋亡率、细胞增殖指数，Western blot法检测血管内皮生长因子（VEGF）和血管内皮生长因子受体-2（VEGFR-2）的表达，实时荧光定量PCR法检测半胱氨酸蛋白酶-3（Caspase-3）和半胱氨酸蛋白酶-9（Caspase-9 ）RNA的表达。结果：与对照组比较，模型组的细胞凋亡率升高[（4.32±0.11）%比（0.34±0.01）%，P<0.05]，细胞增殖指数下降[(43.40±4.04)%比(63.76±2.71)%，P<0.05]，VEGF表达下调（0.09±0.03比0.85±0.06，P<0.05），VEGFR-2表达下调（0.13±0.03比0.81±0.05，P<0.05），Caspase-3 RNA表达上调（1.67±0.02比0.79±0.01，P<0.05 ），Caspase-9 RNA表达上调（2.29±0.02比0.71±0.01，P<0.05）；与模型组比较，天麻组、法半夏组的细胞凋亡率下降[（2.51±0.45)% 、(2.12±0.13)%比(4.32±0.11)%，P<0.05]，细胞增殖指数升高[（50.83±2.46)%、(51.41±2.86)%比(43.40±4.04)%，P<0.05]，VEGF表达上调（0.60±0.04、0.30±0.03比0.09±0.03，P<0.05），VEGFR-2表达上调（0.55±0.05、0.55±0.03比0.13±0.03，P<0.05），Caspase-3 RNA表达下调（1.33±0.03、1.16±0.02比1.67±0.02 ，P<0.05），Caspase-9 RNA表达下调（1.86±0.09、1.82±0.02比2.29±0.02，P<0.05）；与天麻组比较，法半夏组的细胞凋亡率下降[（2.12±0.13)%比(2.51±0.45)%，P<0.05]，细胞增殖指数差异无统计学意义[（51.41±2.86)%比(50.83±2.46)%，P>0.05）]，VEGF表达下调（0.30±0.03比0.60±0.04，P<0.05），VEGFR-2表达差异无统计学意义（0.55±0.03比0.55±0.05，P>0.05），Caspase-3 RNA表达下调（1.16±0.02比1.33±0.03，P<0.05），Caspase-9 RNA表达差异无统计学意义（1.82±0.02比1.86±0.09，P>0.05）。结论：天麻和法半夏均能抑制ox-LDL诱导的HUVECs损伤，其机制可能与上调VEGF和VEGFR-2的表达、下调Caspase-3 RNA和Caspase-9 RNA的表达有关。

英文摘要:

Abstract: Objective: To investigate the effects of Gastrodia elata and Rhizoma pinelliae preparatum on ox-LDL-induced injury of human umbilical vein endothelial cells(HUVECs). Methods：HUVECs were cultured in vitro and randomly divided into control group, model group, Gastrodia elata group (5 mg/mL), Rhizoma pinelliae preparatum group (10 mg/mL), flow cytometry detection of HUVECs cell apoptosis rate, cell proliferation index, Western blot method Detection of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor-2 (VEGFR-2) expression, real-time fluorescent quantitative PCR method to detect cysteine protease-3 (Caspase-3) and cysteine protease- 9 (Caspase-9) RNA expression. Result：Compared with the control group, the apoptosis rate of the model group increased (4.32±0.11 vs. 0.34±0.01, P<0.05), the cell proliferation index decreased (43.40±4.04 vs. 63.76±2.71, P<0.05), and the expression of VEGF was down-regulated ( 0.09±0.03 vs. 0.85±0.06, P<0.05), VEGFR-2 expression was down-regulated (0.13±0.03 vs. 0.81±0.05, P<0.05), Caspase-3 RNA expression was up-regulated (1.67±0.02 vs. 0.79±0.01, P<0.05) ), the expression of Caspase-9 RNA was up-regulated (2.29±0.02 vs. 0.71±0.01, P<0.05). Compared with the model group, the apoptosis rate of Gastrodia elata group and Rhizoma pinelliae preparatum group decreased (2.51±0.45, 2.12±0.13 vs. 4.32±0.11, P<0.05), and cell proliferation index increased (50.83±2.46, 51.41±2.86 vs. 43.40±4.04, P<0.05), the expression of VEGF was up-regulated (0.60±0.04, 0.30±0.03 vs. 0.09±0.03, P<0.05), and the expression of VEGFR-2 was up-regulated (0.55±0.05, 0.55±0.03 vs. 0.13±0.03, P< 0.05), Caspase-3 RNA expression was down-regulated (1.33±0.03, 1.16±0.02 vs. 1.67±0.02, P<0.05), Caspase-9 RNA expression was down-regulated (1.86±0.09, 1.82±0.02 vs. 2.29±0.02, P<0.05) .Compared with the Gastrodia elata group, the apoptosis rate of the Rhizoma pinelliae preparatum group decreased (2.12±0.13 vs. 2.51±0.45, P<0.05), and the cell proliferation index was not statistically different (51.41±2.86 vs. 50.83±2.46, P>0.05). VEGF expression was down-regulated (0.30±0.03 vs. 0.60±0.04, P<0.05), VEGFR-2 expression was not significantly different (0.55±0.03 vs. 0.55±0.05, P>0.05), Caspase-3 RNA expression was down-regulated (1.16±0.02 vs. 1.33±0.03, P<0.05), the expression of Caspase-9 RNA was not statistically different (1.82±0.02 vs 1.86±0.09, P>0.05). Conclusion: Both Gastrodia and Pinelliae can inhibit the HUVEC damage induced by ox-LDL. The mechanism may be related to the up-regulation of the expression of VEGF and VEGFR-2, and the down-regulation of the expression of Caspase-3 RNA and Caspase-9 RNA.

查看全文查看/发表评论下载PDF阅读器

关闭