| 郑增光,王鹏雁.盐酸小檗碱抑制TLR4的表达对恶性黑素瘤细胞增殖、凋亡和迁移的影响[J].浙江中西医结合杂志,2021,31(1): |
| 盐酸小檗碱抑制TLR4的表达对恶性黑素瘤细胞增殖、凋亡和迁移的影响 |
| Effect of berberine hydrochloride on proliferation, apoptosis and migration of malignant melanoma cells by inhibiting the expression of TLR4 |
| 投稿时间：2020-06-10 修订日期：2020-09-25 |
| DOI： |
| 中文关键词: 盐酸小檗碱 黑色素瘤 增殖 凋亡 迁移 TLR4信号通路 |
| 英文关键词:berberine malignant melanoma proliferation apoptosis migration TLR4 signaling pathway |
| 基金项目:浙江省卫生科技计划面上项目(2018252439) |
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| 中文摘要: |
| 目的 探讨盐酸小檗碱对A375黑素瘤细胞增殖、凋亡和迁移的影响。方法 将黑素瘤A375细胞株根据盐酸小檗碱浓度分为4组：对照组（0μmoL／L）、低剂量组（40μmoL／L）、中剂量组（80μmoL／L）、高剂量组（120μmoL／L）。然后使用CCK8法检测各组细胞增殖情况，流式细胞仪检测细胞凋亡情况，通过Transwell法检测各组细胞的迁移能力，同时使用RT-PCR和Western blot检测A375细胞TLR4 mRNA及蛋白表达水平。采用SPSS22.0软件进行统计分析，多组间数据比较采用单因素方差分析，两组间比较采用t检验。结果 盐酸小檗碱（0、40、80、120μmoL／L）分别处理A375细胞后，随盐酸小檗碱浓度的增加，A375细胞存活率（分别为100.00±4.76、70.37±3.06、48.33±0.64、26.60±2.67）逐渐降低（F=298.78,P<0.01）；细胞凋亡率逐渐升高（F=101.76,P<0.01）；细胞迁移力（分别为331.67±7.64、248.67±8.08、155.33±5.03、109.33±9.02）逐渐降低（F=510.81,P<0.01）；同时 TLR4 mRNA及蛋白表达量逐渐降低(p<0.05)。结论 盐酸小檗碱可以促进黑素瘤A375细胞凋亡、抑制A375细胞的增殖和迁移能力，且这种作用与盐酸小檗碱存在剂量-依赖关系。同时其抗肿瘤机制可能与抑制TLR4信号通路有关。 |
| 英文摘要: |
| Objective To explore the effect of berberine hydrochloride on proliferation, apoptosis and migration of A375 melanoma cells. Methods The A375 cell lines of malignant melanoma were divided into 4 groups according to berberine hydrochloride concentration: control group (0 moL/L), low dose group (40 moL/L), middle dose group (80 moL/L) and high dose group (120 moL/L). Cell proliferation in each group was detected by CCK8 assay, apoptosis by flow cytometry, migration ability of cells in each group was detected by Transwell assay； Meanwhile, mRNA and protein expression levels of TLR4 in A375 cells were detected by RT-PCR and Western blot. SPSS22.0 software was used for statistical analysis, one-way anova was used for data comparison between multiple groups, and t test was used for comparison between two groups. Results After treatment with berberine hydrochloride (0, 40, 80, 120 moL/L), the survival rate of A375 cells were(100.00±4.76)%,( 70.37±3.06)%, (48.33±0.64)%,( 26.60±2.67)%,respectively. decreased gradually (F=298.78,P<0.01).The apoptosis rate increased gradually (F=101.76,P<0.01). Cell migration ability (331.67±7.64, 248.67±8.08, 155.33±5.03, and 109.33±9.02, respectively) decreased gradually (F=510.81, P<0.01). At the same time, the protein and mRNA expression levels of TLR4 were significantly decreased, and these changes were dose-dependent with berberine hydrochloride.. Conclusion Berberine hydrochloride can promote the apoptosis of melanoma A375 cells and inhibit the proliferation and migration of A375 cells. Meanwhile, its anti-tumor mechanism may be related to the inhibition of TLR4 signaling pathway. |
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