| 张璐,傅丹青.汉黄芩素对糖尿病肾病大鼠的治疗作用及肾组织RAGE、S100A8表达的影响[J].浙江中西医结合杂志,2020,30(8): |
| 汉黄芩素对糖尿病肾病大鼠的治疗作用及肾组织RAGE、S100A8表达的影响 |
| Therapeutic effect of wogonin on diabetic nephropathy rats and its effects on RAGE and S100A8 expression in renal tissue |
| 投稿时间：2020-05-11 修订日期：2020-06-05 |
| DOI： |
| 中文关键词: 汉黄芩素 糖尿病肾病 晚期糖基化终末产物受体 S100A8 |
| 英文关键词:Wogonin Diabetic nephropathy Receptor for advanced glycation end products S100A8 |
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| 中文摘要: |
| 目的 探讨汉黄芩素对糖尿病肾病大鼠的治疗作用及肾组织晚期糖基化终末产物受体(RAGE)、髓样相关蛋白8(S100A8)表达的影响。方法 SD大鼠60只随机分为对照组、模型组、厄贝沙坦组(15mg/kg)、汉黄芩素低、高剂量组(50、100mg/kg),每组12只；模型组、厄贝沙坦组、汉黄芩素低、高剂量组建立糖尿病肾病大鼠模型,厄贝沙坦组、汉黄芩素低、高剂量组于建模成功后第1天开始灌胃给予相应药物,持续给予8周,对照组和模型组给予等体积生理盐水,试验结束后,测定24h尿蛋白、血肌酐(Scr)、尿素氮(BUN)、血糖,观察大鼠肾脏病理改变,采用实时荧光定量PCR(RT-PCR)法及蛋白免疫印迹(WB)法测定肾脏RAGE、S100A8 mRNA和蛋白水平。结果 模型组大鼠BUN、Scr、24h尿蛋白、血糖、肾脏组织RAGE、S100A8 mRNA和蛋白水平高于对照组(P<0.05)；厄贝沙坦组、汉黄芩素低、高剂量组大鼠BUN、Scr、24h尿蛋白、血糖、肾脏组织RAGE、S100A8 mRNA和蛋白水平低于模型组(P<0.05),且随着汉黄芩素剂量的增加,汉黄芩素低、高剂量组大鼠BUN、Scr、24h尿蛋白、血糖、肾脏组织RAGE、S100A8 mRNA和蛋白水平降低(P<0.05)；汉黄芩素低、高剂量组大鼠BUN、Scr、24h尿蛋白、血糖、肾脏组织RAGE、S100A8 mRNA和蛋白水平高于厄贝沙坦组(P<0.05)。结论 汉黄芩素对大鼠糖尿病肾病具有明显治疗效果；其机制可能与汉黄芩素可对糖尿病肾病大鼠肾脏组织RAGE、S100A8 mRNA和蛋白产生抑制作用有关。 |
| 英文摘要: |
| Objective: To investigate the therapeutic effect of wogonin on diabetic nephropathy rats and its effects on receptor for advanced glycation end products(RAGE) and S100A8 expression in renal tissue. Methods: Sixty SD rats were random divided into control group, model group and irbesartan group (15 mg/kg), low and high dose groups of wogonin (50, 100 mg/kg). The rats in the model group, irbesartan group, low-dose and high-dose groups of wogonin were used to establish diabetic nephropathy rat model; 12 in each group. The rats in the irbesartan group, low-dose and high-dose groups of wogonin were given corresponding drugs by gavage on the first day after successful modeling, and continued to be given for 8 weeks. The rats in the control group and model group were given equal volume of saline. After the end of the experiment, 24h Urine protein, serum creatinine(Scr), blood urea nitrogen(BUN) and blood glucose were measured, and renal pathological changes in rats were observed, and RAGE, S100A8 gene and protein levels renal tissue were determined by Real-time fluorescence quantitative PCR(RT-PCR) and western blot(WB). Results: The levels of BUN, Scr, 24h urinary protein, blood glucose, renal tissues RAGE, S100A8 mRNA and protein in the model group were higher than those in the control group(P<0.05). The levels of BUN, Scr, 24h urinary protein, blood glucose, renal tissues RAGE, S100A8 mRNA and protein in the Irbesartan group, the low and high dose groups of wogonin were lower than those in the model group(P<0.05), and with the increase of wogonin dose, the BUN, Scr, 24h urinary protein, blood glucose, renal tissues RAGE, S100A8 mRNA and protein levels in the low and high dose groups of wogonin were gradually decreased(P<0.05). The BUN, Scr, 24h urinary protein, blood glucose, renal tissues RAGE, S100A8 in the low and high dose groups of wogonin were higher than those in irbesartan group(P<0.05). Conclusion: Wogonin has obvious therapeutic effect on diabetic nephropathy in rats. The mechanism may be related to the inhibitory effect of wogonin on RAGE, S100A8 mRNA and protein in the kidneys of diabetic nephropathy rats. |
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