| 陈芳,施琳琳,王峻,詹程胹,刘敏,陶丽媛,陈冻伢.理气通便合剂对便秘型肠易激综合征模型大鼠结肠、下丘脑5-HT、VIP的影响[J].浙江中西医结合杂志,2021,31(2): |
| 理气通便合剂对便秘型肠易激综合征模型大鼠结肠、下丘脑5-HT、VIP的影响 |
| Effects of Liqi Tongbian Mixture on 5-HT and VIP in colon and hypothalamus of constipation irritable bowel syndrome model rats |
| 投稿时间：2020-04-01 修订日期：2020-06-26 |
| DOI： |
| 中文关键词: 理气通便合剂，便秘型肠易激综合征，5-羟色胺，血管活性肠肽 |
| 英文关键词:Liqi Tongbian Mixture, Constipation-predominant irritable bowel syndrome, 5-hydroxytryptamine, Vasoactive intestinal peptide |
| 基金项目:杭州市农业与社会发展科研计划项目(20170533B69)；杭州市科技发展计划社会发展科研自主申报项目(20180533B74) |
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| 中文摘要: |
| 目的 通过观察理气通便合剂对便秘型肠易激综合征(Constipation-predominant irritable bowel syndrome, IBS-C)模型大鼠结肠、下丘脑5-羟色胺（5-hydroxytryptamine, 5-HT）、血管活性肠肽（Vasoactive intestinal peptide, VIP）的影响，探讨理气通便合剂对IBS-C大鼠的可能作用机制。方法 将SD大鼠24只随机分为空白组，IBS-C模型组，理气通便合剂治疗组，莫沙必利治疗组，采用冰水灌胃法制备IBS-C模型。造模至7天后，予加用药物干预，中药治疗组予理气通便合剂（18.75g·kg-1·d-1）灌胃，西药组予莫沙必利水溶液（1.35mg·kg-1·d-1）灌胃，空白对照组及IBS-C模型组给予等量蒸馏水灌胃。21天后取各组大鼠下丘脑及结肠组织，酶联免疫法（ELISA）检测5-HT、VIP的水平。结果 与空白组相比，模型组大鼠结肠5-HT（35.94±2.06ng/g 比 25.88±3.10ng/g）、下丘脑5-HT（71.12±5.88ng/g 比 50.85±3.14ng/g）、结肠VIP （656.37±60.34pg/g 比 577.86±53.55pg/g）、下丘脑VIP（386.57±38.00pg/g 比 276.17±43.73pg/g）均明显升高(P＜0.05)，药物干预后，中药组及西药组大鼠结肠及下丘脑5-HT、VIP水平较模型组有所下降，中药组较模型组有统计学差异(P＜0.05)，而西药组无明显统计学差异。结论 理气通便合剂的治疗作用可能是通过抑制5-HT、VIP，调节肠道运动及分泌功能，从而达到治疗的作用。 |
| 英文摘要: |
| Abstract: Objective To Observe the effect of Liqi Tongbian Mixture on 5-HT, VIP of Colon and Hypothalamus in IBS-C model rats. Methods Twenty-four SD rats were randomly divided into control group, IBS-C model group, Liqi Tongbian mixture treatment group, and mosapride group. IBS-C model was prepared by ice-water gavage. The Chinese medicine treatment group was administered with Liqi Tongbian mixture (18.75g·kg-1·d-1), the western medicine group was administered with mosapride aqueous solution (1.35mg·kg-1·d-1), and the control group and IBS-C model group was given an equal amount of distilled water. After 21 days, the hypothalamus and colon tissues of each group were taken, and the expressions of 5-HT and VIP were detected by enzyme-linked immunoassay (ELISA). Results Compared with the control group, the levels of 5-HT in the colon and hypothalamus of the model group were increased (35.94±2.06ng/g vs 25.88±3.10ng/g, 71.12±5.88ng/g vs 50.85±3.14ng/g); meanwhile, the same trend was observed on VIP (656.37±60.34pg/g vs 577.86±53.55pg/g, 386.57±38.00pg/g vs 276.17±43.73pg/g). After the drug intervention, the levels of 5-HT and VIP in the colon and hypothalamus of the rats in the traditional Chinese medicine group and western medicine group were higher, and the Chinese medicine group has a statistical difference compared with the model group, while the western medicine group has no significant statistical difference. Conclusion The therapeutic effect of Liqi Tongbian Mixture may be achieved by inhibiting 5-HT and VIP, regulating intestinal movement and secretory function. |
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