| 叶雪丹,顾露囡,王增.NGR修饰唑来膦酸长循环脂质体制备及其表征研究[J].浙江中西医结合杂志,2020,30(11): |
| NGR修饰唑来膦酸长循环脂质体制备及其表征研究 |
| Study of preparation and in vitro characterization of NGR-PEG-ZOL-LPs |
| 投稿时间：2020-02-25 修订日期：2020-03-24 |
| DOI： |
| 中文关键词: 唑来膦酸，长循环，NGR多肽，脂质体，表征 |
| 英文关键词:Zoledronic acid,Long-circulating ,NGR,Liposomes,Characterization |
| 基金项目:浙江省医药卫生科技计划（2017KY263），浙江省自然科学基金项目（LYY18H300001） |
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| 中文摘要: |
| 目的:制备具有肿瘤血管靶向的NGR修饰唑来膦酸长循环脂质体,并对其进行表征研究。方法：采用薄膜分散法制备NGR修饰唑来膦酸长循环脂质体,,并对其进行表征研究,并测定包封率。结果：制备得到的NGR-PEG-ZOL-LPs平均粒径为(271.43±9.23)nm,Zeta电位为-(36.20±0.20)mv,多分散系数(PDI)为(0.15±0.03),理化性质相对稳定；NGR-PEG-ZOL-LPs包封率为8.41%；电镜下显示NGR-PEG-ZOL-LPs呈类圆形,大小分布均匀,无明显聚集。结论：采用薄膜分散法制备的NGR修饰唑来膦酸长循环脂质体具有较高的稳定性,从而为体内药效学研究奠定基础。 |
| 英文摘要: |
| Aim:Preparation of NGR-PEG-ZOL-LPs, and evaluation its characteristics in vitro.Method:Film dispersion method was used to prepare the NGR-PEG-ZOL-LPs, then,their characterization studies including the encapsulation efficiency were performed to determine. Results: The average particle size of the prepared NGR-PEG-ZOL-LPs was (271.43±9.23) nm, the Zeta potential was-(36.20±0.20) mv, and the polydispersity coefficient (PDI) was (0.15±0.03). The physical and chemical properties were relatively stable;the encapsulation rate of NGR-PEG-ZOL-LPs was 8.41%;the electron microscopy showed that NGR-PEG-ZOL-LPs had a round shape, uniform size distribution, and no obvious aggregation. CONCLUSION: NGR-modified zoledronic acid long-circulating liposomes prepared by the thin-film dispersion method have high stability, which lays the foundation for pharmacodynamic research in vivo. |
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