| 张抒,王梦蕾,郑益志.姜黄素对VEGF诱导下HaCaT细胞增殖及miR-203的表达研究[J].浙江中西医结合杂志,2020,30(9): |
| 姜黄素对VEGF诱导下HaCaT细胞增殖及miR-203的表达研究 |
| The Effect of Curcumin on the Proliferation and MiR-203 Expression of HaCaT Cells Induced by VEGF |
| 投稿时间：2020-02-11 修订日期：2020-05-13 |
| DOI： |
| 中文关键词: 姜黄素，银屑病，miR-203，HaCaT细胞，VEGF |
| 英文关键词:Curcumin, Psoriasis, miR-203, HaCaT cell, VEGF |
| 基金项目:浙江省自然科学(No.LY14H110003) |
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| 中文摘要: |
| 目的 通过观察姜黄素对血管内皮生长因子（VEGF）刺激干预后的HaCaT细胞增殖及细胞内微小RNA-203（miR-203）表达的影响，探讨姜黄素治疗银屑病目的的可能作用机制。方法 使用CCK-8法分别检测不同浓度姜黄素（0、19.5、39、78.1、156.2、312.5、625、1250、2500、5000ng/ml)对25ng/ml VEGF刺激干预后的HaCaT细胞增殖的影响。通过实时荧光定量PCR法（Real-time PCR法）检测25ng/ml VEGF、1380ng/ml姜黄素、5000ng/ml姜黄素分别及联合作用下对HaCaT细胞内miR-203表达情况的影响。结果 姜黄素在实验浓度范围内呈浓度依赖性抑制HaCaT细胞增殖，2500ng/ml姜黄素明显抑制细胞增殖。与空白组比较，miR-203在25ng/ml VEGF诱导下的HaCaT细胞中表达明显上调（P＜0.05），而5000ng/ml姜黄素能明显抑制HaCaT细胞中miR-203的表达（P＜0.05），同样能下调25ng/ml VEGF刺激干预下HaCaT细胞中miRNA-203的表达（P＜0.05）。结论 抑制角质形成细胞的过度增殖是姜黄素治疗银屑病的作用机制之一，上述作用机制可能是姜黄素通过下调细胞内miR-203的表达来实现的。 |
| 英文摘要: |
| Objective To explore the possible mechanism of curcumin in the treatment of psoriasis by observing the effect of curcumin and vascular endothelial growth factor (VEGF) on cell proliferation and microRNA-203(miR-203) expression of HaCaT cell. Methods Using CCK8 assay to detect the influence of curcumin with different concentrations（0、19.5、39、78.1、156.2、312.5、625、1250、2500、5000ng/ml) on the proliferation of HaCaT cells induced by 25ng/ml VEGF；25ng/ml VEGF, 1380ng/ml curcumin and 5000ng/ml curcumin were treated cells either independently or together, then real-time PCR assay was used to detect the expression of miR-203 on the cells. Result Curcumin inhibited the proliferation of HaCaT cells in a concentration-dependent manner in the range of 0~5000ng/ml. 2500ng/ml curcumin inhibited the cell proliferation significantly(P＜0.05). Compared with the blank control group, 25ng/ml VEGF promoted the expression of miR-203 in HaCaT cells (t=17.47，P =0.0033), while 5000ng/ml curcumin significantly inhibited the expression(t=17.83，P =0.0031) and decreased the effect of 25ng/ml VEGF(t=22.49，P =0.002). Conclusion One of the mechanisms in the treatment of psoriasis with curcumin is inhibiting excessive proliferation of keratinocytes. The mechanism maybe achieved by the decrease of miR-203 expression in cells. |
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