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邵敏敏.茵陈草水提物对四氯化碳所致肝纤维化大鼠的干预作用[J].浙江中西医结合杂志,2020,30(7):
茵陈草水提物对四氯化碳所致肝纤维化大鼠的干预作用
The effect of Artemisia dracunculus L. water extract on carbon tetrachloride-induced liver fibrosis in rats
投稿时间:2020-01-06  修订日期:2020-05-22
DOI:
中文关键词:  茵陈草水提物  肝纤维化  四氯化碳  大鼠
英文关键词:Artemisia dracunculus L. water extract  liver fibrosis  carbon tetrachloride  rat
基金项目:
作者单位E-mail
邵敏敏 温州医科大学附属慈溪医院慈溪市人民医院 ylwangyuanrong@sina.com 
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中文摘要:
      目的: 研究茵陈草水提物对四氯化碳所致肝纤维化大鼠干预作用的影响。方法:通过向SD大鼠腹腔注射四氯化碳与花生油混合溶液所诱导建立肝纤维化大鼠模型。将肝纤维化模型的大鼠随机分为5组:模型组、阳性药水飞蓟素40mg.kg-1组、茵陈草水提物2.5、5、10 g.kg-1组,每组10只。连续灌胃给药56天,每日1次。另设10只SD大鼠为空白组。模型组、空白组每日灌胃等体积生理盐水。末次给药4 h后,3%苯巴比妥钠腹腔注射麻醉后,腹主动脉取血,离心提取血清。拨离肝脏,HE染色法及Masson染色法观察大鼠肝脏的病理学变化和纤维化程度。试剂盒检测大鼠血清中谷草转氨酶(AST)、丙氨酸转氨酶(ALT)的含量, ELISA法检测肝组织中炎症因子肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)的含量。试剂盒法检测肝脏中透明质酸(HA),III型前胶原(PCIII),层粘连蛋白(LN)、IV型胶原(IV-C)的含量。结果: 与模型相比,茵陈草水提物可降低肝纤维化大鼠血清AST、ALT的水平以及炎症因子TNF-α、IL-1β的含量(P<0.05),肝脏胶原HA、PCIII、LN、IV-C的含量降低(P<0.05)。结论: 茵陈草水提物对四氯化碳所致肝纤维化大鼠肝脏有一定保护作用,其作用机制可能与调节肝组织中炎症因子TNF-α、IL-1β和胶原HA、PCIII、LN、IV-C含量有关。
英文摘要:
      Objective: To investigate the effect of Artemisia dracunculus L. water extract on carbon tetrachloride-induced liver fibrosis in rats. Methods: SD rats were intraperitoneally injected with mixed solution of carbon tetrachloride and peanut oil to established liver fibrosis models. Liver fibrosis rats were randomly divided into 5 groups with 10 animals per group: model control group, silymarin positive control group (40mg.kg-1), Artemisia dracunculus L. water extract groups (2.5、5、10 g.kg-1). Animals were intragastric administration for 56 days, once a day. Ten healthy SD rats were considered as normal control. Model and normal control rats were given equal volume saline. Four hours after last administration, rats were intraperitoneal anesthesia by 3% phenobarbital sodium. We take blood from abdominal aorta and extract serum by centrifugation. Liver samples were collected for HE and Masson staining to observe pathological alterations and fibrosis. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) serum contents were determined. Inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in liver tissues were detected by ELISA method. The hyaluronic acid (HA), type III procollagen (PCIII), laminin (LN) and type IV collagen (IV-C) were also investigated. Results: Compared with model control, Artemisia dracunculus L. water extract could decreased the levels of AST and ALT in serum and contents of TNF-α and IL-1β in inflammatory factors in liver fibrosis rats (P <0.05), as well as the liver collagen HA, PCIII, LN and IV-C. Conclusion: The protective effect of Artemisia dracunculus L. water extract on carbon tetrachloride-induced liver fibrosis in rats is associated with regulating inflammatory factors TNF-α, IL-1β and collagen HA, PCIII, LN, IV-C in liver tissue.
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