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郑祥,王审.桃红四物汤促进自噬抑制氧糖剥夺诱导的心肌细胞凋亡的实验研究[J].浙江中西医结合杂志,2020,30(7):
桃红四物汤促进自噬抑制氧糖剥夺诱导的心肌细胞凋亡的实验研究
The Expermental Research of Taohong Siwu Tang in Promoting Autophagy to Inhibit Myocardial cell apoptosis
投稿时间:2020-01-04  修订日期:2020-04-17
DOI:
中文关键词:  心肌细胞  桃红四物汤  氧糖剥夺  凋亡  自噬
英文关键词:
基金项目:浙江省中医药科技计划项目(2019ZB054),浙江省医药卫生科技计划项目(2018KY136)
作者单位E-mail
郑祥 浙江中医药大学附属温州中西医结合医院 wzzxyys@126.com 
王审 浙江中医药大学附属第二医院 wangshenxnk@163.com 
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中文摘要:
      目的 探讨桃红四物汤(TST)对氧糖剥夺(OGD)诱导的H9C2心肌细胞损伤的保护作用。 方法 将H9C2心肌细胞分为5组(n=3):对照组、OGD模型组、OGD +TST含药血清处理组、OGD +TST含药血清+雷帕霉素处理组、OGD +TST含药血清+氯奎处理组。四唑盐(MTT)比色法检测细胞活力;流式细胞术检测细胞凋亡;免疫印迹法(western blot)检测自噬相关蛋白(LC3、p62)和凋亡相关蛋白(Bax、Bcl-2)的表达。结果 与对照组相比,OGD模型组细胞活力显著下降(P<0.05),细胞凋亡显著增多(P<0.05),LC3II/LC3I比值和促凋亡蛋白Bax显著增多,p62和抗凋亡蛋白Bcl-2表达减少。与OGD模型组相比,OGD +TST含药血清处理组的细胞活力显著升高(P<0.05),细胞凋亡明显减少(P<0.05),LC3II/LC3I比值、Bcl-2表达增多,p62和Bax蛋白表达减少。与OGD +TST含药血清处理组相比,OGD +TST含药血清+雷帕霉素处理组的细胞活力进一步升高(P<0.05),细胞凋亡比例进一步减少(P<0.05),LC3II/LC3I比值、Bcl-2表达进一步增多,p62和Bax蛋白表达进一步减少;而氯奎处理桃红四物汤治疗组的细胞活力下降(P<0.05),凋亡细胞比例升高(P<0.05),LC3II/LC3I比值、Bcl-2表达减少,p62和Bax蛋白表达增多。结论 桃红四物汤促进氧糖剥夺H9C2心肌细胞自噬,抑制心肌细胞凋亡,对心肌缺血起保护作用。
英文摘要:
      Objective To investigate the protective effect of Taohong Siwu Tang(TST) on H9C2 cardiomyocytes injury induced by oxygen and glucose deprivation(OGD). Methods H9C2 cardiomyocytes were randomly divided into 5 groups (n=3): control group, OGD model group, OGD + serum containing TST treatment group, OGD + serum containing TST + rapamycin treatment group, OGD + serum containing TST + cloquinone treatment group. Cell viability was detected by MTT. Apoptosis was detected by flow cytometry. The expression of autophagy related proteins (LC3, p62) and apoptotic related proteins (Bax, Bcl-2) were detected by western blot. Results Compared with the control group, the cell viability of the OGD model group was decreased (P<0.05), the cell apoptosis was increased (P<0.05), the LC3II/LC3I ratio and Bax were significantly increased (P<0.05), p62 and Bcl-2 were decreased (P<0.05). Compared with the OGD model group, we found that the cell viability of the OGD + serum containing TST treatment group was increased (P<0.05), the cell apoptosis was decreased (P<0.05), the LC3II/LC3I ratio and Bcl-2 were increased (P<0.05), p62 and Bax were decreased (P<0.05). Compared with the OGD + serum containing TST treatment group, we found that the cell viability of the OGD + serum containing TST + rapamycin treatment group was increased (P<0.05), the cell apoptosis was reduced (P<0.05), LC3II/LC3I ratio and Bcl-2 was further increased(P<0.05), p62 and Bax was reduced(P<0.05); while in the OGD + serum containing TST + cloquinone treatment group, the cell viability was significantly decreased (P<0.05), the proportion of apoptotic cells was increased (P<0.05), the LC3II/LC3I ratio and Bcl-2 was decreased(P<0.05), p62 and Bax was increased(P<0.05). Conclusion TST promotes OGD of cardiac autophagy, inhibits the cardiac apoptosis, and plays a protective role in myocardial ischemia.#$NL【Keywords】 Myocardial cell;Taohong Siwu Tang; oxygen and glucose deprivation; apoptosis;autophagy
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