| 徐露,张鑫杰,张闰哲,姚庆华.姜黄素逆转结肠癌细胞耐药的实验研究[J].浙江中西医结合杂志,2020,30(6): |
| 姜黄素逆转结肠癌细胞耐药的实验研究 |
| Experiment on Curcumin Reversing Drug Resistance in Colon Cancer Cells |
| 投稿时间：2019-12-10 修订日期：2020-04-28 |
| DOI： |
| 中文关键词: 姜黄素 IL-6/STAT3 细胞凋亡 耐药 |
| 英文关键词:curcumin IL-6/STAT3 Cell apoptosis Drug resistance |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
|
| 摘要点击次数: 445 |
| 全文下载次数: 4 |
| 中文摘要: |
| 目的：探索姜黄素逆转人结肠癌耐药细胞株HCT-116/5FU作用及机制。方法：姜黄素、5-FU诱导细胞凋亡,采用MTT法检测细胞增殖以明确最佳实验条件；AnnexinV-FITC试剂盒检测细胞凋亡率；Western-blot分析耐药细胞株内cleaved-Caspase3、IL-6、STAT3蛋白表达水平。结果：姜黄素能逆转人结肠癌耐药细胞株HCT-116/5FU的耐药程度；对照组、5-FU组、姜黄素组、联合用药组凋亡率分别为(2.5±2.1)%、(6.5±3.2)%、(19.9±7.2)%和(56.0±12.1)%；耐药细胞株中IL-6、STAT3蛋白表达明显高于亲本株；姜黄素的使用降低了耐药细胞株中IL-6、STAT3的蛋白表达量。结论：姜黄素能逆转人结肠癌耐药细胞株HCT-116/5FU的耐药,其机制可能与下调IL-6/ STAT3有关。 |
| 英文摘要: |
| Objective: To explore the effect and mechanism of the drug-resist reversal caused by curcumin on human colon cancer cell line Hct-116/5FU. Methods: Curcumin and 5-FU was used to induce apoptosis; MTT assay was used to determine cell proliferation and determine the experimental conditions based on the previous results. AnnexinV-FITC kit was used to detect the apoptosis rate. Western-blot was used to evaluate the expression levels of cleaved-Caspase3, IL-6 and STAT3 in drug-resistant cell line. Results: Curcumin can reverse the resistance of human colon cancer cell line HCT-116/5FU; The apoptosis rates of the control group, 5-FU group, curcumin group, and combination drug group were (2.5 ± 2.1)%, (6.5 ± 3.2)%, (19.9 ± 7.2)%, and (56.0 ± 12.1)%, respectively; The expression of IL-6 and STAT3 protein in drug-resistant cell lines was significantly higher than the parental strains; Curcumin reduced the protein expression levels of IL-6 and STAT3. Conclusion: Curcumin can reverse the resistance of human colon cancer resistant cell line HCT-116/5FU, and its mechanism may be related to the down-regulation of IL-6 / STAT3. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
