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张媛,徐红.复方芪苓配方颗粒对尿酸性肾病模型大鼠作用机制研究[J].浙江中西医结合杂志,2020,30(5):
复方芪苓配方颗粒对尿酸性肾病模型大鼠作用机制研究
Research on the Mechanism of Compound Qiling Formula Granules in Reducing Uric in Rats with Uric Acid Nephropathy
投稿时间:2019-12-02  修订日期:2020-02-25
DOI:
中文关键词:  复方芪苓配方颗粒  尿酸性肾病  黄嘌呤氧化酶  肾脏尿酸盐转运体
英文关键词:Compound Qiling formula granules  Uric acid nephropathy  Xanthine oxidase  Renal transporter
基金项目:浙江省杭州市科技发展计划项目(2015KJJ12);全国名老中医药专家传承工作室建设项目(国中医药人教发[2016]42号);
作者单位E-mail
张媛 杭州市中医院 zhangyuan19820428@163.com 
徐红 杭州市中医院 xuhong@vip.163.com 
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中文摘要:
      目的 在氧嗪酸钾盐和腺嘌呤诱导大鼠产生的尿酸性肾病模型上,研究复方芪苓配方颗粒降尿酸及改善肾功能的作用机制。方法 SD大鼠随机分为正常对照组、模型对照组、别嘌醇组、复方芪苓配方颗粒高、中、低剂量组。分别测定其血清肌酐(Scr)、尿素(Urea)、尿酸(UA)及肝脏黄嘌呤氧化酶(XOD),免疫组化检测肾脏组织有机阴离子转运体1(OAT1)、尿酸盐转运体(URAT1)、有机阳离子转运体2(OCT2)的表达。结果 与模型对照组比较,复方芪苓配方颗粒各组尿酸性肾病大鼠的Scr、Urea、UA水平显著降低;复方芪苓配方颗粒中、高剂量组肝脏XOD明显降低,肾脏OAT1和OCT2蛋白的表达显著上调,URAT1蛋白的表达显著下调。结论 复方芪苓配方颗粒能降低尿酸性肾病大鼠的Scr、Urea、UA水平,其作用机制可能与降低大鼠肝脏XOD活性,上调大鼠肾脏中OAT1、OCT2的水平,下调URAT1的水平,从而抑制尿酸生成、并通过增加尿酸盐的分泌及降低肾脏尿酸盐重吸收能力促进尿酸排泄相关。
英文摘要:
      Objective To study the mechanism of compound Qiling formula granules on reducing uric acid and improving renal function in the model of rats with uric acid nephropathy, which were induced by potassium oxyzinate and adenine. Methods The SD rats were randomly divided into normal group, model group, allopurinol group and high, medium and low dose group of compound Qiling formula granules. The levels of serum uric acid (UA), creatine (Scr), urea and liver xanthine oxidase (XOD) were tested respectively. The expression of organic anion transporter 1 (OAT1), uric acid transporter (URAT1) and organic cation transporter 2 (OCT2) were detected by immunohistochemistry. Results Compared with model group, the levels of UA, Scr and Urea of the rats with uric acid nephropathy in the groups of compound Qiling formula granules were significantly lowered; the levels of liver XOD in high and medium dose group of compound Qiling formula granules were reduced obviously, the expressions of OAT1 and OCT2 were up-regulated and the expressions of URAT1 were down-regulated significantly. Conclusion Compound Qiling formula granules can lower the levels of UA, Scr and Urea of the rats with uric acid nephropathy. Its mechanism may be related to the decrease of liver XOD activity, the up-regulation of OAT1&OCT2 and the down-regulation of URAT1 in the kidney of rats, thus decreasing the uric acid formation, increasing the secretion of urate and reducing the renal urate reabsorption capacity to promote the uric acid excretion.
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