| 徐俭朴,何飞,陈园园,韩佳颖.肺泡灌洗液Xpert MTB/RIF、TB-RNA和TB-DNA联合检测对初治涂阴肺结核的快速诊断应用[J].浙江中西医结合杂志,2020,30(8): |
| 肺泡灌洗液Xpert MTB/RIF、TB-RNA和TB-DNA联合检测对初治涂阴肺结核的快速诊断应用 |
| Value of Combined Bronchoalveolar Lavage Fluid Xpert MTB/RIF、TB-RNA and TB-DNA Assay in Rapid Diagnosis of Smear-Negative Pulmonary Tuberculosis |
| 投稿时间：2019-11-18 修订日期：2020-01-07 |
| DOI： |
| 中文关键词: 肺泡灌洗液 GeneXpert实时荧光定量PCR快速检测 涂阴肺结核 |
| 英文关键词:bronchoalveolar lavage fluid Xpert MTB/RIF Smear-Negative Pulmonary Tuberculosis |
| 基金项目:杭州市科技发展计划项目(20171226Y24)；杭州市科技发展计划项目（20191203B126） |
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| 中文摘要: |
| 目的 探讨肺泡灌洗液Xpert MTB/RIF、TB-RNA和TB-DNA联合检测对涂阴肺结核快速诊断的应用价值。方法 选取78例涂阴肺结核患者为观察组,37例排除结核病的患者为对照组,行电子支气管镜检查,取肺泡灌洗液分别进行Xpert MTB/RIF、TB-RNA和TB-DNA检测,比较两组患者三种方法检测阳性率及观察组三种方法联合检测的特异度和灵敏度。结果 观察组肺泡灌洗液中Xpert MTB/RIF、TB-RNA和TB-DNA阳性率均显著高于对照组,差异有统计学意义(P<0.01)。观察组中三种方法联合检测涂阴肺结核的灵敏度88%,特异度58.49%,较Xpert MTB/RIF检测法有统计学差异(P<0.01)。结论 肺泡灌洗液Xpert MTB/RIF、TB-RNA和TB-DNA联合检测技术对涂阴肺结核的诊断具有较高的敏感性和特异性,且时效性好,具有较好应用价值。 |
| 英文摘要: |
| Objective To observe the value of combined brochoalveolar lavage fluid Xpert MTB/RIF、TB-RNA and TB-DNA assay in the rapid diagnosis of smear-negative pulmonary tuberculosis. Methods: The BALF samples were obtained from 78 cases of smear-negative pulmonary tuberculosis (observation group) and 37 non-pulmonary tuberculosis patients (control group). Xpert MTB/RIF detection,TB-RNA and TB-DNA in BALF were performed in two groups, and the positive rates of three detection methods in observation group were compared. Sensitivity and specificity of combined three detection methods in observation group were analyzed. Results Compared with control group, The Xpert MTB/RIF、TB-RNA and TB-DNA positive rates in BALF of observation group were significantly higher than that of control group(P<0.01). In observation group, the sensitivity and specificity of combined three detection methods were 88%, 58.49% ,which were higher than Xpert MTB/RIF detection method(P<0.01) .Conclusion. The combined detection technology of alveolar lavage fluid Xpert MTB/RIF, TB-RNA and TB-DNA has high sensitivity and specificity for the diagnosis of smear negative pulmonary tuberculosis, and has good timeliness and application value. |
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