| 伍旭明.槐杞黄对OVA诱导哮喘大鼠模型嗜酸性细胞凋亡及免疫调节因子的影响[J].浙江中西医结合杂志,2020,30(1): |
| 槐杞黄对OVA诱导哮喘大鼠模型嗜酸性细胞凋亡及免疫调节因子的影响 |
| The effects of Huaiqinghuang on the apoptosis of eosinophilic and immunomodulation factors in asthmatic rats induced by OVA |
| 投稿时间：2019-06-22 修订日期：2019-11-30 |
| DOI： |
| 中文关键词: 槐杞黄，哮喘，嗜酸性细胞，炎症细胞因子，细胞凋亡 |
| 英文关键词:Huaiqihuang , asthma, eosinophil, inflammatory cytokines, apoptosis. |
| 基金项目:T20184828005中药特色技术传承人才培训项目：“十三五”浙江省中医药重点学科建设计划。 |
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| 中文摘要: |
| 目的：探讨槐杞黄对哮喘大鼠模型嗜酸性细胞凋亡、Th1/Th2/Th17免疫调节及相关炎症因子的影响。方法：将SD大鼠随机分成对照组、模型组、地塞米松组、槐杞黄组、槐杞黄联合地塞米松组。卵清蛋白（OVA）致敏激发复制大鼠哮喘模型，连续给药15天后，测定各组血清IgE、IL-5、IL-3、粒细胞巨噬细胞集落刺激因子（GM-CSF）及IL-17水平；肺泡盥洗液进行白细胞计数及分类；病理切片观察肺组织病理变化；实时定量-pcr测定组织中Bcl-2以及Bax，NF-κB相对基因表达。结果：与模型组比较，地塞米松组、槐杞黄组以及两药合用可显著降低哮喘大鼠血清中IgE水平[（57.14±5.66）、（57.87±5.31）、（46.68±6.67）比（84.17±5.41），P<0.05，P<0.05，P<0.01）]。此外，槐杞黄联合地塞米松亦可明显降低哮喘大鼠血清中IL-5 [（23.56±6.60）比（22.96±1.31），P<0.05]、IL-3[（2717.38±128.88）比（2033.76±114.33），P<0.01]、GM-CSF [（6.84±5.54）比（6.20±0.65），P<0.01]及IL-17[（252.25±15.14）比（289.64±21.64），P<0.05]水平。另外，地塞米松组和两药联合用药组可降低哮喘大鼠肺泡盥洗液中白细胞总数[地塞米松比模型组（83.41±4.65）比（169.40±11.18），P<0.05；两药联合比模型组（63.7±6.10）比（169.40±11.18），P<0.05]，以及白细胞中EOS[地塞米松比模型组（1.05±0.02）比（7.01±0.18），P<0.05；两药联合比模型组（0.71±0.04）比（7.01±0.18），P<0.05]和LYM[地塞米松比模型组（7.91±0.09）比（17.91±0.11），P<0.05；两药联合比模型组（8.18±0.23）比（17.91±0.11），P<0.05]比例。病理切片中，给药组均可缓解大鼠肺组织肺泡壁及气道周围炎症细胞浸润的现象。同时，地塞米松组、槐杞黄组以及槐杞黄联合地塞米松组均可明显降低哮喘大鼠肺组织中Bcl-2/Bax mRNA相对表达量比值[（0.92±0.24）、（1.65±0.28）、（0.78±0.44）比（6.08±2.31）P<0.01]。并且，槐杞黄组以及槐杞黄联合地塞米松组可降低哮喘大鼠肺组织中NF-κB mRNA相对表达量[（1.19±0.11）、（1.10±0.15）比（1.46±0.08）P<0.05]。结论：槐杞黄辅助治疗可一定程度上减轻哮喘大鼠气道炎症，其机制可能与其促进嗜酸性细胞凋亡，纠正Th1/Th2/Th17免疫失衡，减少哮喘发作炎症因子的水平相关。 |
| 英文摘要: |
| Objective: to investigate the effects of Huaiqihuang on the apoptosis of eosinophil, Th1/Th2/Th17 immunomodulation and related inflammatory factors. Methods: the SD rats were randomly divided into control group, model group, dexamethasone group, Huaiqihuang group, Huaiqihuang joint dexamethasone group. The mouse model of rat asthma was stimulated by egg albumin, and the rats were killed by the rats after 15 days of continuous treatment. The serum IgE, IL-5, IL-3, granulocyte macrophage colony stimulating factor (GM-CSF) and IL-17 levels were determined. The alveolar washing liquid was used to count the white blood cells, and the washing liquid was stained with differential leukocyte count. Lung tissue biopsy was performed to observe pathological changes of lung tissue. The relative gene expression of Bcl-2 and Bax, NF- kappa B in the tissues were determined by real-time quantitative - PCR. Results: compared with the model group, dexamethasone group, Huaiqihuang group and two drugs were used to reduce IgE level in serum of asthma rats, ( 57.14±5.66), (57.87±5.31), (46.68±6.67) vs (84.17±5.41)，P<0.05，P<0.05，P<0.01. In addition, the joint dexamethasone of Huaiqihuang could significantly reduce the levels of IL-5 [(23.56±6.60) vs (22.96±1.31)，P<0.05], IL-3 [(2717.38±128.88) vs (2033.76±114.33)]，P<0.01, GM-CSF [(6.84±5.54) vs (6.20±0.65)，P<0.01], and IL-17 [(252.25±15.14) vs (289.64±21.64)，P<0.05] , in the serum of asthmatic rats. What’s more , the dexamethasone and the combination of two drugs groups could reduce the total white blood cells in the alveolar washing fluid of the asthma rats, (83.41±4.65) vs (169.40±11.18)，P<0.05, (63.7±6.10) vs (169.40±11.18)，P<0.05, and the proportion of EOS [(1.05±0.02) vs (7.01±0.18)，P<0.05, (0.71±0.04) vs (7.01±0.18)，P<0.05] and LYM [(7.91±0.09) vs (17.91±0.11)，P<0.05, (8.18±0.23) vs (17.91±0.11)，P<0.05], in white blood cells. In the pathological section, the infiltration of inflammatory cells around the pulmonary alveolar wall and airway was relieved by the administration of the drug administration. At the same time, the ratio of the relative expression of bcl-2/Bax mRNA expression in the lung tissues of asthmatic rats was significantly reduced by the dexamethasone group, Huaiqihuang groups and the combination of two drugs, (0.92±0.24), (1.65±0.28), (0.78±0.44) vs (6.08±2.31), P<0.01 . Furthermore, the relative expression of nf-kappa B mRNA in the lung tissues of asthmatic rats was also reduced in the groups of Huaiqihuang and the combination of two drugs , (1.19±0.11), (1.10±0.15) vs (1.46±0.08), P<0.05. Conclusion: Huaiqihuang adjuvant therapy could relieve asthma airway inflammation in rats, to a certain extent. And its anti-inflammatory mechanism may be related to promoting the eosinophilic cells apoptosis, correcting the Th1/Th2/Th17 immune imbalance, and lowering the levels of inflammatory markers to reduce asthma attacks. |
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