| 陈卓,钱祥,张爱琴,傅晓璇,甄宏徳.基于肠道菌群结构特征研究胰腺癌不同中医证型与预后的相关性[J].浙江中西医结合杂志,2019,29(12): |
| 基于肠道菌群结构特征研究胰腺癌不同中医证型与预后的相关性 |
| Study on the Correlation between Different TCM Patterns of Pancreatic Cancer and Their Prognosis Based on the Characteristics of Intestinal Flora |
| 投稿时间：2019-06-22 修订日期：2019-10-29 |
| DOI： |
| 中文关键词: 胰腺癌，中医证型，肠道菌群，预后 |
| 英文关键词:pancreatic cancer pattern of traditional Chinese medicine intestinal flora prognosis |
| 基金项目:浙江省中医药科技计划项目(2017ZQ0006) |
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| 中文摘要: |
| 目的 研究胰腺癌不同中医证型肠道菌群结构特征与其总生存期的相关性。方法 设立气血瘀滞组、脾虚湿热组、湿热内蕴组及空白对照组，采集研究对象的粪便标本，进行16S rDNA测序，检测各组间肠道菌群的多样性和丰度，通过组间差异分析明确差异菌属。用Log rank法比较胰腺癌三种中医证型的总生存期差异。结果 显著性差异分析结果显示胰腺癌不同中医证型间具有明显差异(p<0.05)的菌属包括了产碱杆菌属（Alcaligenes）、奇异菌属（Atopobium）和弯曲杆菌属（Campylobacter）等在内的16个菌属；组间差异菌属中的纤毛菌属（Leptotrichia）、奇异菌属（Atopobium）、颗粒链菌属（Granulicatella）、链球菌属（Streptococcus）及弯曲杆菌属（Campylobacter）在脾虚湿热组的丰度均值最高，而在湿热内蕴组中则最低（除弯曲杆菌属外）；生存分析显示，气血瘀滞组的中位生存时间为12个月，脾虚湿热组为8个月，湿热内蕴组为18个月，三组的总生存期差异具有统计学意义，p=0.029。结论 脾虚湿热组中与胰腺癌发生发展可能正相关的菌属丰度最高，其预后最差；而湿热内蕴组与胰腺癌发生发展可能正相关的菌属丰度则最低，其预后最好。 |
| 英文摘要: |
| To study the correlation between the structural characteristics of intestinal flora and the prognosis of different TCM patterns of pancreatic cancer. Methods Qi and blood stasis group, spleen deficiency with damp-heat group, exuberant damp-heat group, and blank control group were set up. 16S rDNA was performed to detect the diversity and abundance of intestinal flora in each group, and the genera of bacteria with significant differences between different groups were found. The log rank method was used to compare the difference in overall survival of three TCM patterns of pancreatic cancer. Results Significant difference analysis showed there were 16 genera including Alcaligenes, Anaerococcus, Atopobium, Campylobacter, etc, which had significant differences (p < 0.05) among different TCM patterns of pancreatic cancer. Leptotrichia, Atopobium, Granulicatella, Streptococcus and Campylobacter had the highest mean abundance in the spleen deficiency with damp-heat group, while they had the lowest mean abundance in the exuberant damp-heat group (except Campylobacter). The overall survival analysis showed that the median survival time of the Qi and blood stasis group was 12 months, spleen deficiency with damp-heat group was 8 months, and the exuberant damp-heat group was 18 months. The difference was statistically significant. p=0.029. Conclusion: Spleen deficiency with damp-heat group had worst prognosis, as it had highest mean flora abundance of bacteria which may positively correlated with the occurrence and development of pancreatic cancer. The exuberant damp-heat group had best prognosis, as it had lowest mean flora abundance of bacteria which may positively correlated with the occurrence and development of pancreatic cancer. |
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