| 李希敏,徐露,周璐,邵田娱,梁珍珍.大蒜素对大鼠肾间质纤维化的干预作用及对TGF-β1/Smads信号通路的影响[*][J].浙江中西医结合杂志,2019,29(10): |
| 大蒜素对大鼠肾间质纤维化的干预作用及对TGF-β1/Smads信号通路的影响[*] |
| Effects of allicin on renal interstitial fibrosis and TGF- 1/Smads signal pathway in rats |
| 投稿时间：2019-05-19 修订日期：2019-09-01 |
| DOI： |
| 中文关键词: 大蒜素 肾间质纤维化 TGF-β1/Smads信号通路 |
| 英文关键词:Allicin Renal interstitial fibrosis TGF- 1/Smads signal pathway |
| 基金项目:2017年度浙江省新苗人才计划项目(2017R410035) |
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| 中文摘要: |
| 目的 观察大蒜素对大鼠肾间质纤维化的干预作用，探究其作用与TGF-β1/Smads信号通路的关系。方法 将SD雄性大鼠随机分为3组，采用单侧输尿管结扎术（UUO）建立肾间质纤维化模型，造模后24h予大蒜素组大鼠大蒜素60mg/(kg?d)灌胃，予假手术组和模型组等量生理盐水灌胃。造模后第15日检测3组大鼠的血清肌酐（SCr）、尿素氮（BUN）及24h尿蛋白（U-TP），并处死大鼠，留取肾组织，包埋切片后行Masson染色，观察肾组织的病理结构，运用免疫组化法检测大鼠肾组织中TGF-β1及α-SMA的表达，Western Blot法测定肾组织中p-Smad2和p-Smad3的表达。结果 与模型组相比，大蒜素组大鼠的体质量显著增高［（298.52±14.82）g比（275.53±23.92）g，P<0.05］，肾脏指数显著下降［（0.53±0.15）%比（0.84±0.25）%，P<0.01］，肾脏组织病变程度有所改善，SCr、BUN及U-TP均显著低于模型组［SCr：（48.54±5.03）μmol/L比（55.10±7.50）μmol/L，BUN：（5.56±0.43）mmol/L比（6.25±0.51）mmol/L，U-TP：（1009.87±105.02）mg/L比（1300.57±229.65）mg/L ，P<0.05］，肾组织中TGF-β1、α-SMA、p-Smad2和p-Smad3蛋白的表达均有下调［TGF-β1：（0.227±0.011）比（0.282±0.020），α-SMA：（0.326±0.009）比（0.471±0.012），p-Smad2：（0.195±0.011）比（0.279±0.016），p-Smad3：（0.220±0.020）比（0.699±0.076），P<0.01］。结论 大蒜素能够通过调节TGF-β1/Smads信号通路，从而改善大鼠的肾间质纤维化程度，对肾功能起到一定的保护作用。 |
| 英文摘要: |
| Objective To observe the intervention effect of allicin on renal interstitial fibrosis in rats and explore the relationship between its effect and the TGF- 1/Smads signal pathway. Methods SD male rats were randomly divided into 3 groups, and the renal interstitial fibrosis model was established by unilateral ureteral obstruction (UUO). After 24 hours of modeling, allicin group was given 60mg/(kg?d) allicin by gavage, and the sham operation group and model group were given the same amount of normal saline by gavage. All rats were sacrificed at 15th day after modeling, the serum creatinine (SCr), urea nitrogen (BUN) and the 24h urine protein (U-TP) were tested, and the obstructed side of kidneys were collected for Masson staining as embedded sections, to observe the pathological structure of kidney tissues. The expression of TGF-β1 and α-SMA in kidney tissue was detected by immunohistochemistry, and the expression of p-Smad2 and p-Smad3 was detected by Western Blot. Results Compared to the model group, the body weight of allicin group was significantly increased [(298.52±14.82)g vs (275.53±23.92)g, P<0.05], and the renal index was significantly decreased [(0.53±0.15)% vs (0.84±0.25)%, P<0.01], and the pathological degree of renal tissue was improved. The SCr, BUN and U-TP of allicin group were significantly lower than the model group [SCr: (48.54±5.03)μmol/L vs (55.10±7.50)μmol/L, BUN: (5.56±0.43)mmol/L vs (6.25±0.51)mmol/L, U-TP: (1009.87±105.02)mg/L vs (1300.57±229.65)mg/L, P<0.05], and the expression of TGF-β1, α-SMA, p-smad2 and p-smad3 in kidney tissues were down-regulated [TGF-β1: (0.227±0.011) vs (0.282±0.020), α-SMA: (0.326±0.009) vs (0.471±0.012), p-Smad2: (0.195±0.011) vs (0.279±0.016), p-Smad3: (0.220±0.020) vs (0.699±0.076), P<0.01]. Conclusion Allicin can improve the degree of renal interstitial fibrosis and play a protective role in renal function by regulating the TGF- 1/Smads signal pathway. |
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