| 翁碧霞,娄江涛,周俊,魏任雄.女贞子多糖在大鼠睾丸支持细胞炎性损伤中对能量代谢保护作用的研究[J].浙江中西医结合杂志,2019,29(4): |
| 女贞子多糖在大鼠睾丸支持细胞炎性损伤中对能量代谢保护作用的研究 |
| The Protective Effect of Ligustri Lucidi Ait Polysaccharide in sertoli cells energy metabolism dysfunction under conditiong of Inflammatory injury |
| 投稿时间：2018-11-29 修订日期：2019-03-16 |
| DOI： |
| 中文关键词: 睾丸支持细胞 氧化损伤 LPS 女贞子多糖 |
| 英文关键词:Sertoli cells Oxidative damage LPS Ligustri Lucidi Ait Polysaccharide |
| 基金项目:浙江省宁波市自然基金项目(2016A610200) |
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| 中文摘要: |
| 目的：探究女贞子多糖(PLL)对LPS诱导的睾丸支持细胞炎性损伤中能量代谢的影响。方法：体外分离和培养大鼠支持细胞,分对照组、LPS组、LPS+低剂量PLL组、LPS +中剂量PLL组和LPS +高剂量PLL组。CCK-8检测细胞增殖；紫外分光光度法检测细胞培养液上清中ATP、LD 含量和LDH、琥珀酸脱氢酶(SDH)活力。结果 各组细胞增殖组间差异有统计学意义(F=152.30,P<0.01),LPS组与对照组相比细胞增殖率明显降低(P<0.01),而中剂量和高剂量LPS+PLL组与LPS组相比细胞增殖率增高 (P<0.01)；各组细胞ATP和LD含量组间差异均有统计学意义(F值分别为57.44和52.31,P均<0.01),LPS组与对照组相比ATP和LD含量均明显降低(P均<0.01)。中剂量和高剂量PLL+LPS组与LPS组相比ATP和LD含量均有不同程度升高,(P<0.05或<0.01)；各组细胞LDH、SDH活力组间差异均有统计学意义(F值分别为41.95和107.43,P均<0.01),LPS组与对照组相比LDH、SDH活力均明显降低(P均<0.01)。中剂量和高剂量PLL+LPS组与LPS组相比LDH、SDH活力均有不同程度升高,(P<0.05或<0.01)结论 女贞子多糖在LPS诱导睾丸支持细胞炎症反应过程中对细胞代谢具有保护作用. |
| 英文摘要: |
| 0bjective: To explore the efect of Ligustri Lucidi Ait Polysaccharide in Sertoli Cells Inflammatory injury Induced by LPS．Methods: The rats sertoli cells were separated in vitro and primarily cultured, and then grouping it with control group、LPS group 、LPS +low does LLP group、LPS+middle does LLP group and LPS+ high does LLP group. The cell proliferation was detected by CCK-8; LDH、SDH activity and ATP、LD concentration in cell culture medium was detected by Ultraviolet spectrophotometry。Results: The difference of cells proliferation in all groups was obvious(F=153.93,P<0.01). Compared with control group, The cell proliferation of LPS group was reduced (P<0.01), and compared with LPS group , these of LPS+ middle does LLP group and LPS+ high does LLP group were raised (P<0.01, respective)；The difference of ATP、LD concentration and LDH、SDH activity in groups was obvious(F=57.44、52.31、41.95 and 107.43,P<0.01,respective), Compared with control group, LPS group’s ATP、LD concentration and LDH、SDH activity were reduced (P<0.01,respective). compared with LPS group, middle and high does LPS + LLP group’s ATP、LD concentration and LDH、SDH activity were gradual raised (P<0.05 or 0.01) Conclusion: Ligustri Lucidi Ait Polysaccharide plays a protective role in sertoli cells energy metabolism dysfunction under conditiong of oxidative damage induced by LPS. |
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