| 沈云飞,詹建伟.槐耳清膏联合顺铂对人肺癌A549细胞和A549/DDP细胞的Bax、Bcl-2以及Survivin基因表达的影响[J].浙江中西医结合杂志,2019,29(2): |
| 槐耳清膏联合顺铂对人肺癌A549细胞和A549/DDP细胞的Bax、Bcl-2以及Survivin基因表达的影响 |
| Effect of Huaier Combined with Cisplatin on Expression of Bax, Bcl-2 and Survivin Genes in Human Lung Cancer A549 Cells and A549/DDP Cells |
| 投稿时间：2018-09-13 修订日期：2018-12-08 |
| DOI： |
| 中文关键词: 槐耳清膏 顺铂 人肺癌细胞A549 人肺癌细胞A549/DDP 癌基因 |
| 英文关键词:Huaier cisplatin human lung cancer cell A549 human lung cancer cell A549/DDP oncogene |
| 基金项目:浙江中医药科技计划项目(2015ZB080) |
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| 中文摘要: |
| 目的 研究槐耳清膏与顺铂联合使用对人肺癌细胞A549和A549/DDP的Bax、Bcl-2以及Survivin基因表达的影响。方法 以体外培养的A549和A549/DDP细胞株为模型,将槐耳清膏进行梯度稀释后与12μg/mL的顺铂联合使用作用于A549细胞和A549/DDP细胞,并以未使用药物刺激作为空白对照组,分别采用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)和酶联免疫吸附法(Enzyme-Linked ImmunoSorbent Assay , ELISA)检测Bax、Bcl-2以及Survivin基因在A549细胞和A549/DDP细胞中的表达情况。结果 槐耳清膏与顺铂联合使用的情况下,随着槐耳清膏浓度的升高,A549细胞和A549/DDP细胞中Bax基因的mRNA的表达逐渐升高,Bcl-2、Survivin基因的mRNA的表达逐渐降低,差异均有统计学意义(P＜0.05)。槐耳清膏与顺铂联合使用的情况下,随着槐耳清膏浓度的升高,A549细胞和A549/DDP细胞中Bax蛋白的表达量逐渐升高,Bcl-2、Survivin蛋白的表达逐渐降低,差异均有统计学意义(P＜0.05)。结论 槐耳清膏与顺铂联合使用能够显著提高人肺癌细胞A549和A549/DDP的Bax基因的表达,降低Bcl-2、Survivin基因的表达。 |
| 英文摘要: |
| Objective To study the effect of Huaier combined with cisplatin on the expression of Bax, Bcl-2 and Survivin genes in human lung cancer cell lines A549 and A549/DDP. Methods The A549 and A549/DDP cell lines were cultured in vitro. The gradient dilution Huaier and 12 μg/mL of cisplatin were added into A549 cells and A549/DDP cells. The cells untreated drugs were set as blank control. The expression of Bax, Bcl-2 and Survivin genes in human lung cancer cell lines A549 and A549/DDP were detected by RT-PCR and ELISA. Results The expression of Bax mRNA in A549 cells and A549/DDP cells increased gradually with the increase of the concentration of Huaier, and Bcl-2 and Survivin mRNA decreased with the increase of the concentration of Huaier, the difference was significant (P<0.05). The expression of Bax protein in A549 cells and A549/DDP cells increased gradually, and the expression of Bcl-2 and Survivin protein decreased gradually with the increase of the concentration of Huaier, the difference was statistically significant (P <0.05). Conclusion The combination of Huaier and cisplatin could significantly increase the expression of Bax gene, decrease the expression of Bcl-2 and Survivin genes. |
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