| 周丽娜.中药活性成分青蒿琥酯增强顺铂对前列腺癌细胞的杀伤活性及机制研究[J].浙江中西医结合杂志,2019,29(6): |
| 中药活性成分青蒿琥酯增强顺铂对前列腺癌细胞的杀伤活性及机制研究 |
| Effect and mechanical research of artesunate on enhancing the cisplatin-induced cytotoxicity against prostate cancer cells |
| 投稿时间：2018-08-13 修订日期：2019-02-14 |
| DOI： |
| 中文关键词: 青蒿琥酯 Met 顺铂 前列腺癌 凋亡 |
| 英文关键词:artesunate Met cisplatin prostate cancer apoptosis |
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| 中文摘要: |
| 目的 探讨体外联用青蒿琥酯对顺铂抗前列腺癌活性的影响并研究其机制。方法: 前列腺癌细胞系LNCaP的细胞活力抑制率用噻唑蓝(MTT)法进行检测。LNCaP细胞的凋亡用流式细胞术进行检测。LNCaP细胞中Met的表达水平,AKT和Bad的磷酸化水平,caspase-9和caspase-3的活化水平用western blot实验进行检测。Bad与Bcl-xl及Bcl-2的相互作用用免疫共沉淀法进行检测。结果: 青蒿琥酯联合顺铂组LNCaP的细胞活力抑制率(61.4±4.9)%和凋亡率(34.8±2.9)%均显著高于顺铂单处理组的细胞活力抑制率(14.7±1.1)%(P<0.05)和凋亡率(12.5±1.0)%(P<0.05)。青蒿琥酯联合顺铂组LNCaP细胞的Met表达水平,AKT和Bad的磷酸化水平均低于顺铂组,而青蒿琥酯联合顺铂组LNCaP细胞的Bad与Bcl-xl及Bcl-2的结合水平,caspase-9和caspase-3的活化水平均均高于顺铂组。青蒿琥酯发挥对顺铂的辅助治疗作用依赖于Met的抑制,青蒿琥酯+顺铂+Met质粒组LNCaP的细胞活力抑制率(20.3±1.4)%和凋亡率(15.5±1.2)%均显著低于顺铂+顺铂组的细胞活力抑制率(61.4±4.9)%(P<0.05)和凋亡率(34.8±2.9)%(P<0.05)。结论: 青蒿琥酯通过Met/AKT/Bad途径增强顺铂对前列腺癌细胞的杀伤活性。 |
| 英文摘要: |
| objective To investigate the effect and mechanism of the artesunate on enhancing the cisplatin-induced cytotoxicity against prostate cancer in vitro. Methods MTT assay was performed to detect the inhibitory rate of cell viability in LNCaP cells. Flow cytometry analysis was performed to detect the apoptosis of LNCaP. Western blot analysis was conducted to detect the expression of Met, phosphorylation of AKT and Bad, and activation of caspase-9 and caspase-3 in LNCaP cells. Co-immunoprecipitation assay was performed to evaluate the interaction with Bad and Bcl-2/Bcl-xl. Results Inhibitory rate of cell viability (61.4±4.9)% and apoptotic rate (34.8±2.9)% of LNCaP in artesunate plus cisplatin group was significantly higher than the inhibitory rate of cell viability (14.7±1.1)% (P<0.05) and apoptotic rate (12.5±1.0)% (P<0.05) in cisplatin single treatment group. Expression of Met and phosphorylation of AKT and Bad in artesunate plus cisplatin group was lower than the cisplatin group, whereas the interaction of Bad with Bcl-xl/Bcl-2, and activation of caspase-9 and caspase-3 was higher than the cisplatin group. Adjuvant effect of artesunate on cisplatin was dependent on the inhibition of Met. Inhibitory rate of cell viability (20.3±1.4)% and apoptotic rate (15.5±1.2)% of LNCaP in artesunate + cisplatin + Met plasmid group was significantly lower than the inhibitory rate of cell viability (61.4±4.9)% (P<0.05) and apoptotic rate (34.8±2.9)% (P<0.05) in artesunate plus cisplatin group. Conclusion Artesunate enhanced the cisplatin-induced apoptosis through the Met/AKT/Bad pathway. |
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