| 沈 雁,王章流,郑华君,钟继红,江向红,徐磊,倪思忆,李思.小檗碱对溃疡性结肠炎小鼠肠上皮细胞凋亡的影响[J].浙江中西医结合杂志,2018,28(12): |
| 小檗碱对溃疡性结肠炎小鼠肠上皮细胞凋亡的影响 |
| Effect of Berberine Hydrochloride on apoptosis of intestinal epithelial cells in mice with Ulcerative Colitis |
| 投稿时间：2018-07-31 修订日期：2018-09-16 |
| DOI： |
| 中文关键词: 小檗碱 溃疡性结肠炎 肠上皮细胞 凋亡 Caspase-12 Caspase-3 |
| 英文关键词:Berberine Ulcerative colitis Intestinal Epithelial Cells Apoptosis Caspase-12 Caspase-3 |
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| 中文摘要: |
| 目的：观察小檗碱（BBR）对溃疡性结肠炎（UC）小鼠肠上皮细胞（IECs）凋亡和Caspase-12、Caspase-3蛋白表达的影响，探讨其治疗UC的可能作用机制。方法：BALB/c小鼠随机分为空白对照组、模型对照组和BBR低、中、高剂量组。采用右旋葡聚糖硫酸钠（DSS）诱导建立UC小鼠模型，同时BBR混悬液灌胃给药7天。观察小鼠一般情况并评估疾病活动指数（DAI），末次给药后结肠组织切片、HE染色并观察组织的病理学变化；TUNEL法检测结肠组织IECs的凋亡情况；免疫组化法测定结肠组织中Caspase-12和Caspase-3蛋白的表达。结果：与模型对照组比较，BBR治疗组小鼠结肠炎的临床症状和组织病理损伤均有较好的改善；模型对照组结肠凋亡细胞显著增多，Caspase-12和Caspase-3蛋白表达显著降低，而BBR治疗后能不同程度地减少细胞凋亡，高剂量BBR治疗能明显下调组织中Caspase-12和Caspase-3的表达水平。结论：BBR能有效缓解UC小鼠的结肠炎症，其机制可能为抑制Caspase-12/Caspase-3凋亡信号通路激活，从而抑制IECs的过度凋亡。 |
| 英文摘要: |
| Objective: To observe the effect of Berberine Hydrochloride(BBR) on apoptosis of intestinal epithelial cells(IECs) and expression of Caspase-12、Caspase-3 in mice with Ulcerative Colitis(UC), and to explore the possible mechanisms of its therapeutic effects on UC. Methods: BALB/c mice were randomly divided into five groups: blank control group, model control group, low dose BBR group, middle dose BBR group and high dose BBR group. UC mice model was established by dextran sulphate sodium(DSS), and daily BBR gavage administration was given for 7 days at the same time. During the course of experiment, the general conditions of mice were observed, and disease activity index (DAI) were assessed. At the end of treatment, colon tissue were sectioned and HE stained, and its pathological changes were observed under light microscope, apoptosis of IECs was determined by TUNEL, the expression level of Caspase-12 and Caspase-3 were detected by immunohistochemical method. Result: Compared with model control group, the clinical symptoms and pathological damage of colitis in BBR groups were well improved. The apoptosis of intestinal epithelial cell was significantly enhanced and the expression level of Caspase-12 and Caspase-3 were greatly decreased in colon tissue in model control group; while BBR treatment could reduce cell apoptosis to various degrees, and high dose BBR treatment could obviously down-regulate the expression level of Caspase-12 and Caspase-3. Conclusion: Berberine can effectively relieve the colitis in UC mice. Its possible mechanism may be ralated to inhibiting excessive apoptosis of IECs by suppressing the activation of Caspase-12/Caspase-3 signal pathway. |
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