| 王英,刘慧芳,楼招欢.抹茶对小鼠实验性急性酒精性肝损伤的干预作用研究[J].浙江中西医结合杂志,2019,29(1): |
| 抹茶对小鼠实验性急性酒精性肝损伤的干预作用研究 |
| Effect of Matcha on mice wiht aute alcoholic liver injury |
| 投稿时间：2018-04-10 修订日期：2018-12-19 |
| DOI： |
| 中文关键词: 茶叶 酒精性肝损伤 抗氧化 |
| 英文关键词:Green tea alcoholic liver injury antioxidate |
| 基金项目:国家自然科学基金青年项目 |
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| 中文摘要: |
| 摘 要 目的 观察抹茶对急性酒精性肝损伤的保护作用。方法 采用灌胃红星二锅头酒连续9天复制急性酒精性肝损伤小鼠模型，造模同时灌胃给予抹茶混悬液。实验期末，采血取血清，全自动生化仪检测血清谷丙转氨酶（ALT）、谷草转氨酶（AST）及碱性磷酸酶（ALP）活性；分离肝脏，计算肝指数，测定肝组织中丙二醛（MDA）水平及超氧化物歧化酶（SOD）活性。结果 抹茶能缓解急性酒精性肝损伤模型小鼠体质量减轻，降低血清ALP活性（模型组116.6±19.4，抹茶组91.9±18.7 U/L）、肝指数（模型组4.62±0.42，抹茶组3.94±0.34 %）及肝组织MDA水平（模型组4.69±0.54，抹茶组3.99±0.75 nmol/gprot），增加SOD活性（模型组19.3±1.00，抹茶组23.5±1.61 U/gprot）。结论 抹茶对急性酒精性肝损伤具有一定的保护作用，该作用可能与其通过增强机体抗氧化能力，抑制脂质过氧化反应有关。 |
| 英文摘要: |
| ABSTRACT Objective To observe the effect of Matcha on experimental aute alcoholic liver injury. Methods ICR mice were orally administered diluted 52% Red Star Erguotou wine for 9 days on end to establish aute alcoholic liver injury model while Matcha suspension was orally administered to the corresponding mice. At the end of the experiment, all the mice were sacrificed and serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels were determined by automatic biochemical analyzer. Livers were harvested and liver index and superoxide dismutase (SOD), malondialdehyde (MDA) levels were measure according to the instruction of the kits. Results Matcha can ease bodyweight reduction of mice caused by over alcohol intake, lower serum ALP level (model116.6±19.4, Matcha 91.9±18.7 U/L) , liver index (model 4.62±0.42，Matcha 3.94±0.34 %) and liver MDA level (model 4.69±0.54, Matcha 3.99±0.75 nmol/gprot) , increase SOD activity (model19.3±1.00, Matcha 23.5±1.61 U/gprot) . Conclusion Matcha possess a protective effect on acute alcoholic liver injury via enhancing antioxidate ability and inhibiting lipid peroxidation reaction. |
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