| 彭心怡,倪锴文,丁阳阳,金红婷,王萍.绞股蓝抗川楝子致小鼠慢性肝损伤的实验研究[J].浙江中西医结合杂志,2018,28(10): |
| 绞股蓝抗川楝子致小鼠慢性肝损伤的实验研究 |
| Experimental study on Gynostemma Pentaphyllum against chronic liver injury induced by Fructus Toosendan in rats |
| 投稿时间：2018-03-25 修订日期：2018-05-09 |
| DOI： |
| 中文关键词: 川楝子 肝毒性 绞股蓝 小鼠 |
| 英文关键词:Fructus Toosendan hepatotoxicity Gynostemma Pentaphyllum rat |
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| 中文摘要: |
| 摘 要 目的 实验研究绞股蓝对川楝子所致慢性肝损伤的保护作用及其机制。方法 将ICR小鼠随机分为正常组、川楝子组、川楝子＋绞股蓝组，各组分别单次灌服蒸馏水、川楝子100g（生药）/kg、川楝子+绞股蓝125g/kg的水提醇沉液。适应性喂养7天后，给药18天，检测血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、计算肝脏指数，测定肝组织超氧化物歧化酶（SOD）、丙二醛（MDA）含量。结果 川楝子组与正常组相比AST显著上升，川绞组AST值与正常组相比无统计学差异且较川楝子组存在下降趋势[(155.17±33.97)比(169.78±55.65)IU/L, P＞0.05]；川绞组MDA含量显著低于正常组，而高于川楝子组[(1.0354±0.20)比(1.4290±0.30)nmol/mg, P＜0.01, 比(0.8018±0.19)nmol/mg, P＜0.05]，SOD/MDA值高于正常组低于川楝子组[(114.98±22.3)比(91.227±18.41)，P＜0.05, 比(156.63±53.65), P＜0.05]；ALT、肝脏指数及SOD方面，三组无显著差异。结论 一定剂量下川楝子连续给药在ICR小鼠上可检测到肝损伤迹象，影响肝细胞内抗氧化系统，绞股蓝与川楝子配伍使用后肝损伤程度下降，降低脂质过氧化产物含量，故绞股蓝有抗川楝子慢性肝损伤的作用，配伍存在临床可行性。 |
| 英文摘要: |
| Objective To investigate the protective effects and mechanism of the Gynostemma Pentaphyllum(GP) against chronic liver injury induced by Fructus Toosendan(FT) in rats. Method Rats were divided into three groups randomly: control group, FT group(100g/kg), FT+ GP group(125g/kg). After seven days of adaptive feeding, all the groups were filled the medicine in stomach for 18 days. At the end of 18 days, the blood was collected and the levels of alanine aminotransferase ( ALT), aspartate aminotransferase (AST) in serum, malondialdehyde (MDA), and superoxide dismutase (SOD) in liver tissues were examined. Result Compared to the control group, the FT group’s level of the serum ALT increased [(169.78±55.65):(120±35.05)IU/L, P＜0.05], the AST of the GP+FT group had no statistically difference, and the GP+FT group has a decrease trend compared with the GP group [(155.17±33.97): 169.78±55.65)IU/L, P >0.05]; the MDA of the GP+FT group was lower than that of the control group (P < 0.01) but higher than that of the GP group(P < 0.05), the SOD/MDA was higher of the GP+FT group than that in the normal group (P < 0.05); there were no significant differences of ALT, liver index and SOD in three groups. Conclusion The results disclose in a given dose, the FT can be detected liver injury in ICR mice, it affects the antioxidant system in cells. It may be possible to reduce the lipid peroxidation of liver tissue with the compatibility of FT and GP, which has clinical feasibility |
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