| 戴卓睿,姜凯.槲皮素通过SIRT1/ROS/DR5途径发挥对TRAIL的协同抗前列腺癌活性[J].浙江中西医结合杂志,2018,28(10): |
| 槲皮素通过SIRT1/ROS/DR5途径发挥对TRAIL的协同抗前列腺癌活性 |
| Quercetin exerts synergistic effect on TRAIL-induced cytotoxicity against prostate cancer through the SIRT1/ROS/DR5 pathway |
| 投稿时间：2017-11-15 修订日期：2018-09-18 |
| DOI： |
| 中文关键词: 槲皮素 TRAIL 前列腺癌 SIRT1 ROS DR5 |
| 英文关键词:quercetin TRAIL prostate cancer SIRT1 ROS DR5 |
| 基金项目: |
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| 中文摘要: |
| 目的: 探讨中药活性成分槲皮素是否对TRAIL有协同抗前列腺癌效应并研究其机制。方法: MTT实验检测前列腺癌细胞系PC3的细胞活力；流式细胞术检测PC3细胞的凋亡和ROS的产生；western blot实验检测PC3细胞中SIRT1、DR5的表达水平及caspase-8、caspase-3的活化水平。结果: TRAIL联合槲皮素对PC3的细胞活力抑制率(64.7±5.2)和凋亡诱导率(34.7±2.6)显著高于TRAIL单治疗组的细胞活力抑制率(16.9±1.4,P<0.05)和凋亡诱导率(9.1±0.8,P<0.05)。TRAIL联合槲皮素治疗组的SIRT1表达水平显著低于TRAIL单治疗组,同时TRAIL联合槲皮素治疗组的DR5表达水平、ROS产生水平及caspase-8、caspase-3活化水平均显著高于TRAIL单治疗组。另外,TRAIL+槲皮素+SIRT1质粒组PC3的细胞活力抑制率(23.4±1.9)和凋亡诱导率(12.5±1.2)及TRAIL+槲皮素+NAC组PC3的细胞活力抑制率(21.5±1.8)和凋亡诱导率(11.3±1.1)均显著低于TRAIL联合槲皮素组PC3的细胞活力抑制率(64.7±5.2,P<0.05)和凋亡诱导率(34.7±2.6,P<0.05)。结论: 槲皮素通过SIRT1/ROS/DR5途径发挥对TRAIL的协同抗前列腺癌活性。 |
| 英文摘要: |
| AIM: To investigate the synergistic effect and mechanism of quercetin on increasing the cytotoxicity of TRAIL against prostate cancer. Methods: MTT assay was performed to test the cell viability of PC3. Flow cytometry analysis was performed to detect the apoptosis and production of ROS in PC3 cells. Western blot analysis was performed to evaluate the expression of SIRT1 and DR5, and the activation of caspase-8 and caspase-3 in PC3 cells. Results: Cell viability inhibitory rate (64.7±5.2) and apoptotic rate (34.7±2.6) of PC3 cells in TRAIL plus quercetin group was significantly higher than the cell viability inhibitory rate (16.9±1.4, P<0.05) and apoptotic rate (9.1±0.8, P<0.05) of PC3 cells in TRAIL single treatment group. Expression of SIRT1 in the TRAIL plus quercetin group was significantly lower than that in the TRAIL group. Meanwhile, expression of DR5, generation of ROS, and activation of caspase-8 and caspase-3 in the TRAIL plus quercetin group was significantly higher than that in the TRAIL single treatment group. In addition, cell viability inhibitory rate (23.4±1.9) and apoptotic rate (12.5±1.2) of PC3 in TRAIL+quercetin+SIRT1 plasmid group and cell viability inhibitory rate (21.5±1.8) and apoptotic rate (11.3±1.1) of PC3 in TRAIL+quercetin+NAC plasmid group was significantly lower than the cell viability inhibitory rate (64.7±5.2, P<0.05) and apoptotic rate (34.7±2.6, P<0.05) of PC3 in TRAIL+quercetin group. Conclusion: Quercetin exerts synergistic effect on TRAIL-induced cytotoxicity against prostate cancer through the SIRT1/ROS/DR5 pathway. |
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