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冯兴华.HBV感染慢性肝病患者体内microRNA-29表达水平及临床价值[J].浙江中西医结合杂志,2018,28(9):
HBV感染慢性肝病患者体内microRNA-29表达水平及临床价值
The expression and clinical value of micro RNA-29 in patients with the hepatitis B virus related chronic liver diseases
投稿时间:2017-11-07  修订日期:2018-07-13
DOI:
中文关键词:  【关键词】微小RNA-29  肝炎,乙型,慢性  临床价值
英文关键词:[Key words] micro RNA-29  Hepatitis B, Chronic  clinical value
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作者单位E-mail
冯兴华 台州恩泽医疗中心(集团)恩泽医院 fengxh6122@enzemed.com 
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中文摘要:
      【摘要】目的:评估微小RNA(microRNA)-29在乙型肝炎病毒(HBV)感染慢性肝病患者体内的表达水平及临床价值。方法:选取我院治疗170例HBV感染慢性肝病患者作为研究对象,根据临床诊断分为:慢性HBV携带(ASC)组35例、慢性乙型肝炎(CHB)组80例、乙型肝炎肝硬化(LC)组30例及肝癌(HCC)组25例,其中CHB组根据病情程度再分为轻度25例、中度30例、重度25例。另选择同期健康体检者30例作为对照组。实时荧光定量PCR法测定血清microRNA-29含量。应用Pearson相关分析血清microRNA-29表达水平与血清ALT、HBsAg、HBV DNA以及AFP水平的相关性。结果:各组患者血清microRNA-29相对表达量存在差异,HC组及ASC组> CHB组> LC组>HCC组(P均<0.05);在CHB组的三个亚组也存在差异,重度>中度>轻度(P均<0.05)。应用Pearson相关分析,CHB组与LC组患者血清microRNA-29相对表达量与血清HBV DNA以及HBsAg水平呈负性相关(P均<0.05);HCC组患者血清microRNA-29相对表达量与AFP血清水平呈负相关(r=-0.817,P=0.013);ASC组、CHB组、LC组及HCC组血清microRNA-29相对表达量与血清ALT水平均无相关关系(P均>0.05)。结论:血清microRNA-29的表达水平与CHB的肝损伤及HCC发生有关。 【关键词】微小RNA-29;肝炎,乙型,慢性;临床价值
英文摘要:
      [Abstract] Objective: To analyze the expression and clinical value of micro RNA-29 in patients with the hepatitis B virus (HBV) related chronic liver diseases. Methods: The 170 patients diagnosed to the HBV related chronic liver diseases were chosen to analyze and were divided into four groups according to clinical diagnosis, which were ASC group (35 cases, diagnosed to hepatitis B virus carriers), CHB group (80 cases, diagnosed to chronic hepatitis B), LC group (30 cases, diagnosed to 1iver cirrhosis) and HCC group (25 cases, diagnosed to hepatocellular carcinoma). Then the patients of CHB group were divided into three sub-groups, which were mild sub-group (25 cases), moderate sub-group (30 cases), and severe sub-group (25 cases). The contemporaneous 30 healthy controls were also chosen to HC group. The serum micro RNA-29 levels were assessed by real-time fluorescece quantitative PCR (RT-PCR). The correlations between serum micro RNA-29 levels and serum indexes (including ALT, HBsAg, HBV DNA and AFP) were analyzed by Pearson relativity analysis. Results: The serum micro RNA-29 levels were different among all groups, which were HC group and ASC group > CHB group > LC group >HCC group (all P <0.05). And the serum micro RNA-29 levels were different among the three sub-groups of CHB group, which were severe sub-group > moderate sub-group > mild sub-group (all P <0.05). The negative correlations between serum micro RNA-29 levels and the serum HBV DNA and HBsAg levels in CHB group and LC group were confirmed by Pearson relativity analysis (all P <0.05). The negative correlations between serum micro RNA-29 levels and the serum AFP levels in HCC group were confirmed (r=-0.817, P=0.013). But there were no correlations between serum micro RNA-29 levels and the serum ALT levels in ASC group, CHB group, LC group and HCC group (all P >0.05). Conclusion: The relativity of serum micro RNA-29 levels and the HBV related liver damage and the pathogenesis of liver cancer were confirmed.  [Key words] micro RNA-29; Hepatitis B, Chronic; clinical value
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