| 蒋珍凤,王恩智,童夏生.模式识别受体 RIG-I和MDA-5在婴幼儿呼吸道感染中表达的意义[J].浙江中西医结合杂志,2019,29(2): |
| 模式识别受体 RIG-I和MDA-5在婴幼儿呼吸道感染中表达的意义 |
| Roles of pattern recognition receptor RIG-I and MDA-5 in infant patients with respiratory tract infections. |
| 投稿时间：2017-09-10 修订日期：2017-10-04 |
| DOI： |
| 中文关键词: 模式识别受体 毛细支气管炎 急性上呼吸道感染 呼吸道合胞病毒 流感病毒 |
| 英文关键词:pattern recognition receptor bronchiolitis acute upper respiratory infection respiratory syncytial viruses influenza viruses |
| 基金项目:浙江省温岭市科技局基金资助项目(2015-1-58) |
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| 中文摘要: |
| 【】 目的 检测呼吸道感染婴幼儿血清RIG-I和MDA-5蛋白的表达,探讨模式识别受体在呼吸道合胞病毒感染性毛细支气管炎和流感病毒感染性急性上呼吸道感染疾病发病机制中的作用。方法 标本来自毛细支气管炎、急性上呼吸道感染及健康儿童(对照组)各50例,采用酶联免疫吸附试验法检测血清RIG-I和MDA-5蛋白的浓度。结果 毛细支气管炎组急性期和急性上呼吸道感染组血清RIG-I和MDA-5蛋白的浓度(依次为14.39±3.97、12.35±4.07 ng/mL；18.78±7.39、16.00±4.50 ng/mL)均显著高于对照组(5.47±1.72 ng/mL；7.34±2.11 ng/mL)(均P<0.01)；毛细支气管炎组急性期血清RIG-I蛋白的浓度(14.39±3.97 ng/mL)显著高于急性上呼吸道感染组(12.35±4.07ng/mL)(P<0.05),两组间MDA-5蛋白的浓度(18.78±7.39 ng/mL；16.00±4.50 ng/mL)差异无统计学意义(P>0.05)。毛细支气管炎组恢复期血清RIG-I和MDA-5蛋白的浓度(10.12±4.13 ng/mL；13.88±6.11 ng/mL)均显著低于急性期(14.39±3.97 ng/mL；18.78±7.39 ng/mL)(均P<0.01)。RIG-I和MDA-5的表达呈显著直线正相关(r=0.40, n=150,P<0.01)。结论 模式识别受体RIG-I和MDA-5在婴幼儿呼吸道感染中表达增加,它们共同参与病毒感染的发病机制,其表达水平随着病情恢复而相应下降。 |
| 英文摘要: |
| Objective To investigate the potential roles of pattern recognition receptor in the pathogenesis of respiratory syncytial viruses infection bronchiolitis, influenza virus acute upper respiratory infection, the concentrations of serum pattern recognition receptor retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) protein were determined. Methods Blood samples were obtained from each 50 cases of bronchiolitis and acute upper respiratory infection and health infant(control group), the concentrations of serum RIG-I and MDA5 protein were assessed using enzyme linked immunosorbent assay (ELISA). Results The concentrations of serum RIG-I and MDA-5 protein were significantly higher in acute infection period of bronchiolitis group and in acute upper respiratory infection group(14.39±3.97、12.35±4.07 ng/mL；18.78±7.39、16.00±4.50 ng/mL，respectively）than those in control group（5.47±1.72 ng/mL；7.34±2.11 ng/mL, respectively）（both P < 0.01）. Moreover, the concentrations of serum RIG-I protein in acute infection period of bronchiolitis group(14.39±3.97 ng/mL) were significantly higher than those in acute upper respiratory infection group(12.35±4.07ng/mL) P<0.05）, whereas, there was no statistical difference of serum MDA-5 protein concentration between in this two groups（18.78±7.39 ng/mL；16.00±4.50 ng/mL）（P>0.05）. Dramaticly, in convalescent period of bronchiolitis group, the concentration of serum RIG-I and MDA-5 protein （10.12±4.13 ng/mL；13.88±6.11 ng/mL）were significantly decreased when compared with acute infection period of bronchiolitis group（14.39±3.97 ng/mL；18.78±7.39 ng/mL）（both P<0.01）. Furthermore, levels of serum RIG-I protein was strongly positive correlated with MDA-5 protein （r=0.40, n=150,P<0.01）. Conclusion Levels of pattern recognition receptor RIG-I and MDA-5 protein were increased in respiratory tract infections of infant patients, the elevated of them indicated that pattern recognition reception participate synergistically in the pathogenesy of viruls infection. Whereas,they were decreased correspondingly when the pathogenetic condition was recovered. |
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