| 吴三英,谭晓霞,毛其芬.黄芩素通过SIRT1/p53途径提高宫颈癌细胞对顺铂的敏感性[J].浙江中西医结合杂志,2018,28(7): |
| 黄芩素通过SIRT1/p53途径提高宫颈癌细胞对顺铂的敏感性 |
| Baicalein increased sensitivity of cervical cancer cells to cisplatin through the SIRT1/p53 pathway |
| 投稿时间：2017-08-10 修订日期：2018-06-01 |
| DOI： |
| 中文关键词: 黄芩素 顺铂 宫颈癌 SIRT1 p53 凋亡 |
| 英文关键词:Baicalein cisplatin cervical cance SIRT1 p53 apoptosis |
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| 中文摘要: |
| 目的: 探讨中药活性成分黄芩素是否对顺铂有协同抗宫颈癌效应并研究其机制。方法: MTT实验检测宫颈癌细胞系Hela的细胞活力；流式细胞术检测Hela细胞的凋亡；western blot实验检测Hela细胞中SIRT1、p53、乙酰化p53、Puma的表达水平及caspase-3的活化水平。结果: 顺铂联合黄芩素对Hela的细胞活力抑制率(66.3±5.7)和凋亡诱导率(38.4±3.2)显著高于顺铂单治疗组的的细胞活力抑制率(18.1±1.4,P<0.05)和凋亡诱导率(10.4±0.9,P<0.05)。顺铂联合黄芩素治疗组的SIRT1表达水平显著低于顺铂单治疗组,同时顺铂联合黄芩素治疗组的p53乙酰化水平和表达水平、Puma表达水平及caspase-3活化水平均显著高于顺铂单治疗组。另外,顺铂+黄芩素+SIRT1质粒组Hela的细胞活力抑制率(25.7±2.1)和凋亡诱导率(14.5±1.1)显著低于顺铂联合黄芩素组Hela的细胞活力抑制率(66.3±5.7,P<0.05)和凋亡诱导率(38.4±3.2,P<0.05)。结论: 黄芩素通过SIRT1/p53途径增强顺铂对宫颈癌细胞的杀伤活性。 |
| 英文摘要: |
| AIM: To investigate the synergistic effect and mechanism of baicalein on increasing the cytotoxicity of cisplatin to cervical cancer. Methods: MTT assay was performed to test the cell viability of Hela. Flow cytometry analysis was performed to detect the apoptosis of Hela cells. Western blot analysis was performed to evaluate the expression of SIRT1, p53, acetylated p53 and Puma, and the activation of caspase-3 in Hela cells. Results: Cell viability inhibitory rate (66.3±5.7) and apoptotic rate (38.4±3.2) of Hela cells in cisplatin plus baicalein group was significantly higher than the cell viability inhibitory rate (18.1±1.4, P<0.05) and apoptotic rate (10.4±0.9, P<0.05) of Hela cells in cisplatin single treatment group. Expression of SIRT1 in the cisplatin plus baicalein group was significantly lower than that in the cisplatin group. Meanwhile, the expression of p53 and Puma, and the activation of caspase-3 were significantly higher in the cisplatin plus baicalein group rather than the cisplatin single treatment group. In addition, cell viability inhibitory rate (25.7±2.1) and apoptotic rate (14.5±1.1) of Hela in cisplatin + Baicalein + SIRT1 plasmid group was significantly lower than the cell viability inhibitory rate (66.3±5.7, P<0.05) and apoptotic rate (38.4±3.2, P<0.05) of Hela in cisplatin + Baicalein group. Conclusion: Baicalein enhanced the cytotoxicity of cisplatin against cervical cancer through the SIRT1/p53 pathway. |
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