| 武瑞,刘寿荣,刘春涛,傅晓晴,王江娅.急性肝衰竭大鼠肝肠功能紊乱的防治研究[J].浙江中西医结合杂志,2018,28(5): |
| 急性肝衰竭大鼠肝肠功能紊乱的防治研究 |
| Prevention and control research on liver intestinal dysfunction in rats with acute hepatic failure. |
| 投稿时间：2017-08-09 修订日期：2018-03-15 |
| DOI： |
| 中文关键词: 大黄 谷氨酰胺 急性肝衰竭 肠道功能紊乱 |
| 英文关键词:Rhubarb Gln Acute hepatic failure Intestinal dysfunction. |
| 基金项目:浙江省中医药科技计划项目(2010ZB123) |
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| 中文摘要: |
| 目的: 观察大黄联合谷氨酰胺对急性肝衰竭大鼠肝功能及肠道屏障功能的防治作用。方法: 1、分组：SD雄性大鼠60只，随机分成3组，肝衰竭模型组20只，对照组20只，大黄联合谷氨酰胺（Gln）组（用药防治组）20只。2、造模：采用腹腔注射法，用D-氨基半乳糖（GalN）对肝衰竭模型组、用药防治组大鼠进行急性肝衰竭（Acute Hepatic Failure，AHF）造模。3、给药：造模前两天，分别给肝衰竭模型组和对照组大鼠予生理盐水灌胃，用药防治组大鼠予大黄煎液和Gln溶液灌胃。4、标本处理及检测：首先，造模后连续4天监测大鼠肝功能，并于96h后对大鼠进行处死，同时对大鼠肝脏炎症的病理变化进行评分；最后，观察大鼠血清中和肠组织中的二胺氧化酶（DAO）水平。结果：用药防治组及对照组大鼠24h、48h、72h的肝功能水平明显优于肝衰竭模型组（P<0.05）。用药防治组及对照组大鼠中肝脏炎症的评分明显低于肝衰竭模型组（p<0.01）。用药防治组及对照组大鼠中的血清DAO水平明显低于肝衰竭模型组（p<0.01）；用药防治组及对照组大鼠中的肠组织DAO水平明显高于肝衰竭模型组（p<0.01）。结论: 大黄能护肝降酶退黄，改善肠道微循环；Gln具有保护肝脏、维持肠道屏障功能等作用。大黄联合Gln能降低AHF大鼠死亡率，对AHF大鼠肠道功能紊乱有防治作用。 |
| 英文摘要: |
| Objective To investigate the effects of rhubarb combined glutamine on liver function and intestinal barrier function in rats with acute hepatic failure（AHF）.Methods 1、Grouping：60 rats were randomly divided into 3 groups: the Hepatic failure model group (n=20), The control group (n=20), prevention and cure of the combination of rhubarb and Gln group(drug control group)(n=20). 2、Modeling: using abdominal injection method, GalN was used to model AHF in rats with hepatic failure model drug and control group.3、Dose：2 days before the mou，The hepatic failure model group and the control group were fed with 0.9% physiological saline，The drug control group were fed with Rhenm Officinal Decoction and 3% solution of Gln. 4、Specimen handling and testing：First, the rat liver function was monitored for 4 days after molding，it was executed after 96h，the pathological changes of liver inflammation in rats were also evaluated，finally, the level of diamine oxidase (DAO) in rat serum and intestinal tissue was observed. Results The hepatic functional level of 24h, 48h and 72h of rats treated with drug control group and control group was significantly better than that of hepatic failure model group（P<0.05）；The scores of liver inflammation in the drug control group and the control group were significantly lower than those in the liver failure model group（p<0.01）；The level of serum DAO in the drug control group and control group was significantly lower than that in the hepatic failure model group（p<0.01）；The DAO level of intestinal tissue in the drug control group and control group was significantly higher than that in the hepatic failure model group（P<0.01）.Conclusions The present results show that rhubarb can protecting liver,reducing?enzyme activity?and eliminating jaundice, improve microcirculation of intestinal. Gln can also protect liver, improve the function of intestinal barrier. The combination of rhubarb and Gln can reduce the mortality in AHF rats and prevent the intestinal dysfunction of AHF rats. |
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