| 汪卫,于健宁,陶筱娟.槐杞黄颗粒对MRL/Lpr狼疮肾小鼠Th17细胞的影响及机制研究[J].浙江中西医结合杂志,2017,27(9): |
| 槐杞黄颗粒对MRL/Lpr狼疮肾小鼠Th17细胞的影响及机制研究 |
| Study on the effect and mechanism of Huai Q Huang Granule on Th17 cells of MRL/Lpr mice with lupus nephritis |
| 投稿时间：2017-05-06 修订日期：2017-06-16 |
| DOI： |
| 中文关键词: 系统性红斑狼疮 槐杞黄 肾损伤 MRL/Lpr狼疮小鼠 Th17细胞 |
| 英文关键词:Systemic lupus erythematosus Huai qi huang renal injury MRL/lpr lupus mice Th17 cells |
| 基金项目:杭州市科技发展计划——医疗卫生及重点专科专病科研攻关专项 |
|
| 摘要点击次数: 1036 |
| 全文下载次数: 2 |
| 中文摘要: |
| 目的:探究槐杞黄颗粒对MRL/Lpr狼疮肾小鼠Th17细胞的影响以及相关机制探讨。方法:正常饲养C57BL/6小鼠8只为空白对照组。8-10周龄MRL/Lpr狼疮小鼠，随机分为MRL/Lpr狼疮小鼠组，槐杞黄颗粒治疗组，地塞米松治疗组。MRL/Lpr狼疮小鼠12周龄左右开始发病，分别给予槐杞黄颗粒或地塞米松治疗，检测各组处理前后24小时尿蛋白定量，血清中TGF-β、IL-17含量，肾脏中RORC mRNA表达水平。 结果:槐杞黄颗粒能降低狼疮小鼠24小时尿蛋白定量水平；通过抑制TGF-β的表达（P<0.001），从而减少了CD4+T 细胞向Thl7细胞分化，使得Th17细胞比例减少（P<0.001）；槐杞黄颗粒治疗后狼疮小鼠肾脏的单核细胞中RORC mRNA的表达均显著下调（P<0.001）。结论:槐杞黄颗粒可以减少狼疮肾小鼠蛋白尿的排出，调节Th17细胞分化，其机制可能与RORCmRNA表达下调有关。 |
| 英文摘要: |
| Objective: To investigate the effect of Huai qi huang（HQH）on Th17 cells of MRL/Lpr mice with lupus nephritis. Methods: Eight normal8-10 weeks C57BL/6 mice were fed as blank control group. MRL/Lpr lupus erythematosus mice were randomly arranged to three groups:MRL/Lpr lupus group, HQH treated group, Dexamethasone(DXM) treated group. After the appearance of clinical symptoms at 12 weeks age, Mrl/lpr mice were treated with HQH or Dexamethasone. The levels of 24 hour urine protein quantitation,TGF-β, IL-17,RORC mRNA and the proportion of TH17 in CD4+ T cells of the each groups before and after treatment were detected. Results: HQH could reduce the level of 24 hours urinary protein,could inhibit the expression of TGF-β(P<0.001) and the percentage of Th17 cells (P<0.001); After the combined treatment with HQH,the expression of RORC mRNA in monocytes from kidney of lupus mice was significantly decreased (P<0.001). Conclusion: HQH can decrease the excretion of proteinuria and regulate the differentiation of Th17 cells, which may be related to the down regulation of RORCmRNA expression. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
