| 胡旻,桑雅清.虎杖中白藜芦醇对人结肠癌HCT-15细胞周期介导的凋亡的研究[J].浙江中西医结合杂志,2017,27(12): |
| 虎杖中白藜芦醇对人结肠癌HCT-15细胞周期介导的凋亡的研究 |
| The mechanism of resveratrol-induced cell cycle mediated apoptosis in Human colon cancer HCT-15 cells |
| 投稿时间：2017-04-19 修订日期：2017-06-20 |
| DOI： |
| 中文关键词: 虎杖,白藜芦醇,细胞周期,凋亡,人结肠癌。 |
| 英文关键词:Polygonum cuspidatum, Resveratrol, cell cycle, apoptosis, human colon cancer. |
| 基金项目: |
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| 摘要点击次数: 838 |
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| 中文摘要: |
| 目的 探讨虎杖中主要药用成分白藜芦醇对人结肠癌HCT-15细胞周期以及增值的影响。
方法 将25，50，100，200 μmol/L 白藜芦醇作用人结肠癌HCT-15细胞，不加药处理作为对照组，采用噻唑蓝(MTT)法检测虎杖中白藜芦醇对HCT-15细胞增殖的影响，在倒置相差显微镜下观察细胞形态变化，流式细胞术检测细胞周期的分布以及细胞凋亡的百分比。免疫印迹方法以及聚合酶链反应法观察主要的细胞周期蛋白以及基因的表达情况。
结果 虎杖中白藜芦醇能显著抑制 HCT-15细胞的生长， 并呈现时间-浓度依赖性。细胞形态学检测显示随着药物浓度的升高，细胞减少皱缩。流式细胞仪结果显示，白藜芦醇作用于细胞HCT-15且细胞被阻滞于S期， 呈现出剂量依赖性。不同药物浓度白藜芦醇(50，100，200 μmol/L) 作用人结肠癌HCT-15细胞48 h后， 随着药物浓度的增大，G0 /G1 期的比例逐渐减小，与对照组比较差异有统计学意义(P<0.05)。而实验组细胞S期的比例随着浓度增大而减小，分别达到(31.07±1.75) %，( 28.66±1.71) %，( 23.65±0.62) %，与对照组(34.89±0.66) % 比较，差异有统计学意义(P<0.05)。免疫印迹法检测显示，白藜芦醇以时间依赖方式下调周期蛋白cyclin D1的表达，上调 p21cip1 蛋白以及细胞凋亡相关蛋白剪切行caspase-3和PRAP的表达。
结论 虎杖中白藜芦醇能明显抑制HCT-15细胞的增殖，并诱导其细胞凋亡，可引起HCT-15细胞的 S 期阻滞。 |
| 英文摘要: |
| Objective: To investigate the effect of resveratrol on the cell cycle and proliferation of human colon cancer HCT-15 cells.
Methods: 25,50,100,200 μmol/L resveratrol was used to treat human colon cancer HCT-15 cells, no treatment as control group. MTT assay was used to detect the effect of resveratrol to the HCT- 15 cell proliferation. Morphological changes were observed under inverted phase contrast microscope. Flow cytometry was used to detect the cell cycle distribution and the percentage of apoptosis. Western blotting and polymerase chain reaction were used to observe the expression of major cell cycle proteins and genes expression.
Results: Resveratrol treatment significantly inhibited the growth of HCT-15 cells with a time and concentration-dependent manner. Cell morphological in a shrinkage manner with the drug concentration increased. Flow cytometry showed that resveratrol blocked the HCT-15 cells in the S phase in a dose-dependent manner. The percentage of G0/G1 phase decreased with the increasing of drug concentration (50,100,200 μmol/L) for 24 h in human colon cancer HCT-15 cells. The difference between the control group was statistically significant (P<0.05). (31.07±1.75) %，( 28.66±1.71) %，( 23.65±0.62) %, respectively, compared with the control group (34.89±0.66)% (P <0.05). The expression of cyclin D1 was up-regulated by resveratrol in a time-dependent manner, as well as the expression of cleaved caspase-3, PARP and p21cip1 proteins and genes.
Conclusion: Resveratrol extracted from Polygonum cuspidatum can inhibit the proliferation of HCT-15 cells and induce the cells to apoptosis, and arrest the cells into S phase. |
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