| 钱家树,林学正,卢光涛.小潮气量高频机械通气增加糖尿病小鼠肺损伤[J].浙江中西医结合杂志,2017,27(10): |
| 小潮气量高频机械通气增加糖尿病小鼠肺损伤 |
| The protective mechanical ventilation strategy based on low tidal volumes and high frequency increases lung injury in diabetes mice QIAN Jiashu,LIN Xuezheng,LU Guangtao. TaiZhou central Hospital(TaiZhou University Hospital) ,Taizhou city ,Zhejiang province ,318000,China |
| 投稿时间：2017-04-07 修订日期：2017-05-25 |
| DOI： |
| 中文关键词: 【关键词】 机械通气 小潮气量 糖尿病 肺损伤 炎症反应 |
| 英文关键词:mechanical ventilation low tidal volume diabetes lung injury inflammation |
| 基金项目: |
|
| 摘要点击次数: 752 |
| 全文下载次数: 1 |
| 中文摘要: |
| 【】 目的 建立糖尿病小鼠模型,运用小潮气量高频机械通气,观察其对炎症反应及肺损伤的影响。方法 40只成年雄性C57BL / 6N小鼠(50～60g)采用随机数字表法分为四组(n=10)：Dia-MV组：糖尿病+机械通气组；Dia-SB组：糖尿病+自主呼吸组；Con-MV组：正常对照+机械通气；Con-SB组：正常对照+自主呼吸。机械通气参数：潮气量为8 ml/kg,频率150次/分,吸入氧浓度0.35。4小时后进行血浆肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量检测。同时,利用RT-PCR对肺组织匀浆炎症因子mRNA的表达进行比较。结果 机械通气前,Dia-MV组和Dia-SB组中的血浆TNF-α,IL-6和IL-10的水平高于Con-MV组和Con-SB组,并且Dia-MV组高于Dia-SB组或Con-MV组或Con-SB组(P <0.05)。与基础值相比, Dia-MV组血浆TNF-α,IL-6和IL-10水平在随着通气持续时间逐渐增加,而Con-SB组中没有发现显着变化。肺匀浆中这些促炎细胞因子的定量RT-PCR分析显示类似血浆的结果(P <0.05)。Dia-MV组中血浆SOD的活性随着机械通气进行降低,MDA水平则相反增加(P <0.05)。在整个研究中, Con-SB组中未发现MDA水平和SOD活性的显著变化。肺匀浆中SOD/MDA定量RT-PCR分析显示类似血浆的结果(P <0.05)。与Con-SB组相比,其他三组肺损伤评分增加：Dia-MV组(3.83±0.34,P <0.05),Dia-SB组(2.11±0.20,P <0.05) ,Con-MV(1.98±0.18,P <0.05)。Dia-MV组中的肺组织可见间质水肿,炎性细胞浸润,肺泡破裂出血,而在Dia-SB组或Con-MV组中仅发现轻微的肺微观结构变化。结论 小潮气量高频机械通气增加糖尿病小鼠肺损伤。 |
| 英文摘要: |
| 【】 Objective The purpose of this present study was to observe the effects of diabetes on inflammation and lung injury in mice with protective ventilation strategy. Methods Forty mice were included in our study. Twenty diabetic were randomized into two groups: Group Dia-MV and Group Dia-SB; another twenty normal mice were randomly divided into Group Con-MV and Con-SB, n=10 in each group. The mice in Group Dia-MV and Con-MV were subjected to 4 hour-ventilation, whose parameters were set as follows: tidal volume 8 ml / kg, frequency 150 breaths / min, and a fraction of inspired oxygen 0.35. And the mice in Group Dia-SB and Con-SB were exposed to room air breathing spontaneously for 4 h. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), superoxide dismutase (SOD) and malondialdehyde (MDA) levels in serum were detected and the expression of inflammatory cytokine mRNA was also determined in lung tissue. Lung damage was assessed using a modified lung injury score. Results The serum levels of TNF-α, IL-6, and IL-10 in Group Dia-MV were significantly higher than those in Group Dia-SB or Group Con-MV or Group Con-SB from first to fourth hour of ventilation (P <0.05). Compared to baseline values, the serum levels of these cytokines were gradually increased over ventilation duration in Group Dia-MV. Quantitative RT-PCR analysis of pro-inflammatory cytokines in lung homogenates presented similar results. The activities of serum SOD decreased and MDA levels were conversely increased along with ventilation duration in Group Dia-MV. The mice in Group Dia-MV suffered obvious lung histological changes, whose lung injury scores were significantly higher in Group Dia-SB as compared to Group Con-SB , Group Con-MV or Group Dia-SB (P<0.05). Conclusion The protective mechanical ventilation strategy based on low tidal volumes and high frequency increased the inflammation reaction and associated lung injury in diabetes mice. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
