| 黄海林,刘俊.银杏叶提取物对内耳组织及蜗神经核线粒体DNA4834bp缺失突变的影响[J].浙江中西医结合杂志,2017,27(10): |
| 银杏叶提取物对内耳组织及蜗神经核线粒体DNA4834bp缺失突变的影响 |
| The effect of the extract of ginkgo biloba on mitochondrial DNA4834bp deletion of inner ear tissue and cochlear nucleus of brain |
| 投稿时间：2017-03-14 修订日期：2017-08-10 |
| DOI： |
| 中文关键词: 银杏叶提取物 氧化损伤 线粒体DNA4834bp 内耳 耳蜗核 |
| 英文关键词:EGB761 Oxidative damage mitochondrial DNA4834bp inner ear cochlear nucleus |
| 基金项目:浙江省中医药管理局编号2009CB017；浙江省卫生厅编号2009B120 |
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| 中文摘要: |
| 目的；研究银杏叶提取物对内耳组织及蜗神经核线粒体DNA4834bp缺失突变的影响。方法：取SD老龄大鼠(24月龄)20只,随机分为2组,老年实验组10只,用银杏叶提取物灌胃治疗,另设老年对照组10只,每天等体积的生理盐水灌胃3月后处死,用药干预前后分别测试ABR,采用荧光定量PCR检测两组大鼠线粒体DNA4834bp缺失率差别,观察线粒体DNA4834bp缺失率与老年听力损失关系。结果：老年实验组(银杏叶组)用药干预前后ABR阈移增加了3.91±3.73 dB peSPL,老年对照组(生理盐水组)ABR阈移增加9.64±2.59 dB peSPL,两者ABR阈移有统计学差异(P＜0.05)。老年实验组蜗神经核线粒体DNA4834bp缺失率较对照组降低了3.2倍,老年实验组内耳组织线粒体DNA4834bp较对照组降低了1.9倍(P＜0.01)。结论：银杏叶提取物能抑制大鼠听觉器官线粒体DNA4834bp缺失突变,改善老年性聋听力,提示银杏叶提取物能减少mtDNA的氧化损伤,改善线粒体功能,从而延缓老年性耳聋进展。 |
| 英文摘要: |
| Objective: To investigate the effect of Extract of Ginkgo Biloba (EGB761) on mitochondrial DNA4834bp deletion of inner ear tissue and cochlear nucleus of brain and to study the related mechanism. Methods：Twenty SD rats at 24 months of age were randomly divided into two group: the experimental group (EGB761 group, n=10), which were received gastric infusion of EGB761 (100mg/kg/d) for 12 weeks. The control group (saline group, n=10). which were infused the same volume saline. Auditory brainstem response (ABR) was used to detect the threshold of rats. The mtDNA4834bp deletion in inner ear and cochlear nucleus of brain was respectively identified by real-time PCR and to be observed the relationship between the ABR threshold shift. Results:1.The difference in ABR threshold shift between experimental group(3.91±3.73 dB peSPL)and control group(9.64±2.59 dB peSPL) was significant(P＜0.05). The mitochondrial DNA4834bp common deletion mutation in cochlear nucleus of experimental group was 3.2 times lower than that in the control group, and the mitochondrial DNA 4834bp in inner ear of experimental group was 1.9 times lower than that in the control group (P＜0.01)
Conclusion: 1. EGB761 can obviously inhibit the increase of level of mitochondrial DNA deletion in inner ear and cochlear nucleus of brain to retard aging in the aged rat by protecting mitochondrial DNA from oxidative damage. |
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