| 岑璐莎.葛根素抑制视网膜新生血管机制的初步研究[J].浙江中西医结合杂志,2017,27(10): |
| 葛根素抑制视网膜新生血管机制的初步研究 |
| A preliminary study on puerarin’s inhibitory effect on retinal neovascularization |
| 投稿时间：2017-02-22 修订日期：2017-05-07 |
| DOI： |
| 中文关键词: 葛根素；视网膜新生血管 VEGF；PECAM-1 |
| 英文关键词:puerarin retinal neovascularization VEGF PECAM-1 |
| 基金项目: |
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| 中文摘要: |
| 目的：研究葛根素对氧诱导大鼠视网膜新生血管VEGF和PECAM-1表达影响，进一步探讨其对新生血管的抑制作用。
方法：建立氧诱导大鼠新生血管模型，并分成空白组，葛根素小剂量组及葛根素大剂量组（每组4只），通过荧光素眼底血管造影观察各组新生血管渗漏区域改变，免疫荧光染色观察PECAM-1表达，ELISA测定玻璃体VEGF表达量。
结果：新生血管渗漏程度、PECAM-1，VEGF表达随葛根素剂量增加而减少，与空白组比较，葛根素小剂量玻璃体液中 VEGF 表达明显降低（ p<0.001)， 且葛根素大剂量组降低程度明显增大(p<0.001)。
结论：葛根素可通过抑制VEGF及PECAM-1表达，抑制氧诱导大鼠视网膜新生血管增生。 |
| 英文摘要: |
| ABSTRACT: Objective: To investigate the effect of puerarin on the expression of VEGF and PECAM-1 on hyperoxia-induced retinopathy in rats. Methods: Rat models of hyperoxia-induced neovascularization were established. Models were divided into blank, small-dose puerarin, and large-dose puerarin groups (n=4, each). We observed the leakage area(using fluorescein fundus angiography，FFA) ,detected the expression of PECAM-1 by immunofluorescence staining and VEGF in vitreum by ELISA. Results: Puerarin administration reduced neovascularization leakage area ,inhibited PECAM-1,VEGF expression in a dose-dependent manner. Compared with blank, The expression of VEGF was decreased (p<0.001) in the small-dose group, and the degree of reduction was significantly increased in the large-dose group (p<0.001). Conclusion: Puerarin can inhibit the hyperoxia-induced retinal neovascularization in rats by inhibiting the expression of VEGF and PECAM-1. |
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