| 徐燕芳,卞银燕,王天游,俞文华,董晓巧.清脑和血方治疗大鼠脑胶质瘤的药效作用及其初步机制研究[J].浙江中西医结合杂志,2017,27(5): |
| 清脑和血方治疗大鼠脑胶质瘤的药效作用及其初步机制研究 |
| Study on therapeutic effects of treatment of rat glioma with prescription of QingNaoHeXue formula and its preliminary mechanism |
| 投稿时间：2016-12-01 修订日期：2016-12-30 |
| DOI： |
| 中文关键词: 清脑和血方 胶质瘤 C6细胞 CREB |
| 英文关键词:QingNaoHeXueformula Glioma C6 cell CERB |
| 基金项目:浙江省中医药科技计划(2013ZB098)；浙江省自然科学基金（Y17H270003） |
|
| 摘要点击次数: 719 |
| 全文下载次数: 1 |
| 中文摘要: |
| 观察清脑和血方对大鼠脑胶质瘤的药效作用，并初步探讨其作用机制，为清脑和血方的临床推广应用提供实验依据。方法 培养鼠源性C6胶质瘤细胞，采用脑立体定位注射C6细胞建立大鼠胶质瘤模型；将成模大鼠随机分为3组：模型对照组、顺铂组、清脑和血方组，每组10只；另设10只大鼠为空白对照组。连续给药25天，观察各组大鼠的一般体征，行为学与体重改变，记录生存率；处死大鼠后取血，分离血清，检测血清中免疫因子IL-2、IL-10、TNF-α、IFN-γ的水平；依次剥离脑组织与胶质瘤组织，称重，计算瘤重占脑组织重百分比；HE染色观察胶质瘤组织病理；免疫印迹法检测胶质瘤组织中CREB的表达情况。结果 成功建立了大鼠胶质瘤模型，相比于空白对照组，模型对照组、顺铂组与清脑和血方组大鼠行为出现明显异常，体重显著下降（P＜0.05，P＜0.01）；四组大鼠的生存率：空白对照组＞清脑和血方组＞模型对照组＞顺铂组；相比于模型对照组，清脑和血方组大鼠的瘤重[(0.0121±0.0050)g]、瘤重占脑组织重百分比[0.49±0.0075]、血清中IL-2[(91.14±10.33)pg/ml]、IL-10[(49.58±6.79)pg/ml]含量、瘤组织中CREB的表达量均明显降低（P＜0.01），血清中IFN-γ[(198.54±11.83)pg/ml]、TNF-α[(209.41±21.82)pg/ml]含量明显升高（P＜0.01），肿瘤组织的病理变化有所减轻，且效果均优于顺铂组。结论 清脑和血方能够明显抑制大鼠C6胶质瘤细胞的颅内生长，其作用机制可能与调节机体免疫因子水平、抑制肿瘤相关蛋白CREB的表达有关。 |
| 英文摘要: |
| To observe the effects of prescription of QingNaoHeXue formula(QNHXf)to rat glioma, and preliminary to discuss the mechanism, providing
an experimental basis for clinical application of prescription of QNHXf. Methods To cultivate rat C6 glioma cells, building rat glioma model by using brain stereotaxic injected C6 cells. The model rats were randomly divided into 3 groups: model control group, cisplatin group, QNHXf group, each group of 10; In addition, using 10 rats as a blank control group. Continuous dosing for 25days, observe each group general signs of rats, behavioral science and weight change, record the life cycle; After put rats to death, get blood, and make a separation of serum, detecting the level of immunologic factors cytokines IL-2, IL-10, TNF-α and IFN-γ in serum. Peel brain tissue and glioma tissues in turn, weighing, calculating the percentage of tumor weight in brain weight; making an observation gliomas histopathologic with HE dyeing; detecting the expression of CREB in glioma tissues by Western Blot. Results Rat glioma model was set up successfully, compared with the blank control group, the behavior of model control group, cisplatin group and QNHXf group appeared abnormal, weight decreased significantly(P<0.05,P<0.01). Survival rate of four groups: blank control group>QNHXf group>model control group>cisplatin group; Compared with model control group, tumor weight[(0.0121±0.0050)g], the percentage of tumor weight in brain weight[0.49±0.0075], the content of IL-2[(91.14±10.33)pg/ml]、IL-10[(49.58±6.79)pg/ml] in serum, the volume expression of CREB in tumor tissue were significantly lower(P<0.01),the content of IFN-γ[(198.54±11.83)pg/ml]、TNF-α[(209.41±21.82)pg/ml]in serum were significantly higher(P<0.01).The pathological changes of tumor tissue were somewhat decreased,and the effect was better than that of cisplatin group.Conclusion The prescription of QNHXf can obviously inhibit the growth of C6 glioma cells in intracranial of rat, its mechanism may be related to regulate the body's immunologic factors levels and inhibit the expression of tumor associated protein CREB. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
