| 钱祥,甄宏德,李永峰,张爱琴.莪术石油醚提取物对三阴性乳腺癌细胞MDA-MB-231的转移相关基因影响的体外研究[J].浙江中西医结合杂志,2017,27(6): |
| 莪术石油醚提取物对三阴性乳腺癌细胞MDA-MB-231的转移相关基因影响的体外研究 |
| Effect of metastasis-associated gene on Triple Negative Breast Cancer Cell with Petroleum Ether Extracts of Curcuma zedoaria in vitro |
| 投稿时间：2016-10-25 修订日期：2017-04-10 |
| DOI： |
| 中文关键词: 莪术石油醚提取物 三阴性乳腺癌细胞 甲基化 细胞转移 |
| 英文关键词:triple negative breast cancer cell petroleum ether extracts of curcuma zedoaria, methylation cell metastasis |
| 基金项目:浙江省中医药科技计划项目(2016ZA034)(2016ZB023) |
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| 中文摘要: |
| 目的：观察莪术石油醚提取物(PECZ)对三阴性乳腺癌细胞增殖、细胞趋化因子及粘附因子的影响。基于表观遗传调控的角度,观察莪术石油醚提取物对三阴性乳腺癌细胞转移相关的甲基化作用。方法：以三阴性乳腺癌细胞株MDA-MB-231作为研究对象,观察莪术石油醚提取物对它的干预作用,设立表阿霉素作为阳性对照组,设立生理盐水作为阴性对照组。采用CCK-8法检测药物对细胞株的增殖抑制作用；采用RT-PCR法检测药物干预前后粘附因子E-cadherin、E-selectin和趋化因子 SDF-1、CXCR4 mRNA的表达变化；采用MS-HRM法检测药物干预前后粘附因子E-cadherin的启动子甲基化水平的变化。结果: 1、与空白对照组相比较,不同浓度的表阿霉素组及PECZ组对细胞增殖均有一定的抑制作用,其差异有统计学意义( P ＜0.05)。2、与空白对照组相比较,300μg/ml浓度的PECZ干预细胞24h后,可降低细胞趋化因子CXCR4 mRNA的表达,提高趋化因子SDF-1 mRNA和细胞粘附因子E-selectin mRNA的表达,并显著提高细胞粘附因子E-cadherin mRNA的表达,其差异有统计学意义( P ＜0.05)。3、与空白对照组、低剂量PECZ组相比,高剂量PECZ组能降低乳腺癌细胞粘附因子E-cadherin启动子的甲基化水平。结论：本研究结果显示莪术石油醚提取物对乳腺癌细胞具有一定的抑制作用,其可显著上调细胞粘附因子E-cadherin mRNA的表达,其对细胞转移和侵袭的抑制作用可能是通过这一途径实现的。在甲基化层面,一定浓度的莪术石油醚提取物对乳腺癌细胞粘附因子E-cadherin抑癌基因的启动子具有一定的去甲基化作用。 |
| 英文摘要: |
| Objective: Observe Petroleum Ether Extracts of Curcuma zedoaria accepting to Triple Negative Breast Cancer Cell MDA-MB-231, including cell proliferation inhibition, cell chemokines, adhesion factor and the mechanism of methylation. Methods: It was assayed by CCK8 for MDA-MB-231 cellular viability with various concentrations, RT PCR analyses for mRNA of chemokines molecules and adhesion molecules, MS-HRM analyses for E-cadherin promoter. Epirubicin and NS were used as control for the study. Results:1.Compared with the blank control group,MDA-MB-231 cells were inhibited by PECZ and Epirubicin(p<0.05).2. Compared with the blank control group, (RT-PCR) analysis demonstrated that the E-cadherin mRNA, SDF-1 mRNA and E-selectin mRNA expression level were increased and the CXCR4 mRNA expression level was decreased 24h after treated with PECZ at the concentrations of 300 μg/mL (P < 0.05). 3.Compared with the blank control group and low-dose group(100ug/ml),high-dose (300ug/ml) PECZ can reduce levels of methylation on adhesion moleculesSE-cadherinSpromoter. Conclusions: This study suggests that PECZ can inhibit the proliferationSof breast cancer cells and inhibit theSMetastasis and invasion of breast cancer cells possiblySby upregulatingSthe expression ofSE-cadherin mRNA. In methylation level, PECZ has someSdemethylation function on E-cadherinSpromoter. |
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