| 严伟伟.铅暴露对小鼠海马凋亡细胞、乙酰胆碱酯酶活性的影响[J].浙江中西医结合杂志,2017,27(1): |
| 铅暴露对小鼠海马凋亡细胞、乙酰胆碱酯酶活性的影响 |
| Effects of lead exposure on the apoptotic cells in the hippocampus and the activity of acetylcholinesterase of mice |
| 投稿时间：2016-08-09 修订日期：2016-10-28 |
| DOI： |
| 中文关键词: 铅 学习记忆 凋亡细胞 乙酰胆碱酯酶 |
| 英文关键词:lead learning and memory apoptotic cells acetylcholinesterase |
| 基金项目:杭州市卫生科技计划（一般）项目（No.2014A41） |
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| 中文摘要: |
| 目的 研究铅暴露对小鼠学习记忆、海马凋亡细胞及乙酰胆碱酯酶（AchE）活性的影响。方法 健康昆明小鼠30只，随机分为对照组（7mg/kg，生理盐水）、醋酸铅暴露组（7mg/kg，醋酸铅），每组15只，连续腹腔注射8天。采用Morris水迷宫实验检测小鼠学习记忆功能，羟胺比色法测定AChE 活性、TUNEL染色检测海马凋亡细胞。结果 Morris水迷宫实验显示，醋酸铅暴露组小鼠第4/5天的逃避潜伏期时间（22.2?4.096s/19.73?2.88s）、穿越平台时间（15.54?3.72s）较对照组（13.64?2.925s/11.79?2.24s、3.24?2.24s）明显延长（P＜0.05）。且醋酸铅暴露组小鼠AChE表达（0.229?0.042 U/ug）、海马凋亡细胞数量较对照组（0.498?0.131 U/ug）显著增高（P＜0.05）。结论 铅暴露可明显损伤小鼠的学习记忆能力，而小鼠海马凋亡细胞的增加、中枢神经系统的Ach含量的降低可能是其学习记忆功能障碍的一个可能机制。 |
| 英文摘要: |
| Objective To study the effects of lead exposure on learning and memory , the apoptotic cells in the hippocampus and the activity of acetylcholinesterase（AchE） of mice. Methods A total of 30 healthy Kunming mice were randomly divided into the control group （7mg/kg，normal saline）and the lead acetate exposure group（7mg/kg，lead acetate）,15 rats in each group and 8 days in continuous intraperitoneal injection. The Morris water maze test was used to detect the learning and memory function of mice, hydroxylamine colorimetric method was used to measure AChE activity, and TUNEL staining was used to detect the apoptotic cells in the hippocampus. Results The Morris water maze test showed that mice exposed to lead acetate group, the escape latency time of mice in the first 4/5 days(22.2?4.096s/19.73?2.88s) and the time of crossing the platform(15.54?3.72s) were significantly longer than those of the control group(13.64?2.925s/11.79?2.24s、3.24?2.24s) (P＜0.05).The expression of AChE(0.229?0.042 U/ug) and the number of apoptotic cells in the hippocampus of the lead acetate exposure group were significantly higher than those in the control group(0.498?0.131 U/ug) (P＜0.05). Conclusion Lead exposure can obviously damage learning and memory ability of mice. The increase of apoptotic cells in the hippocampus of mice and the decrease of the content of Ach in the central nervous system may be a possible mechanism of learning and memory dysfunction. |
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