| 沈英英,武利强,王博,邓姝,林圣云.再障患者B细胞及其介导的体液免疫状况研究[J].浙江中西医结合杂志,2017,27(1): |
| 再障患者B细胞及其介导的体液免疫状况研究 |
| Research of Aplastic Anemia Mechanismabout B Lymphocytesand Humoral Immunity |
| 投稿时间：2016-05-26 修订日期：2016-09-10 |
| DOI： |
| 中文关键词: 再生障碍性贫血,免疫球蛋白,B淋巴细胞亚群 |
| 英文关键词:Aplastic anemia, immunoglobulin, Blymphocyte subsets |
| 基金项目:2015年度国家中医临床研究基地业务建设科研专项（JDZX2015121），2014年度省级公益性技术应用研究计划（2014c33273） |
|
| 摘要点击次数: 779 |
| 全文下载次数: 1 |
| 中文摘要: |
| 目的：检测AA组与对照组的免疫球蛋白(IgG、IgA及IgM)含量以及B淋巴细胞亚群计数(CD20+B淋巴细胞,CD19+CD38+B淋巴细胞,CD5+CD19+淋巴细胞亚群),了解两组的体液免疫表达差异。方法：速率散射比浊法检测AA组与对照组的免疫球蛋白(IgG、IgA及IgM)含量,比较免疫球蛋白的表达情况。流式法检测CD20+B淋巴细胞,CD19+CD38+D的活化B淋巴细胞,CD5+CD19+的B淋巴细胞亚群计数。结果：(1)AA组和对照组的各免疫球蛋白浓度无统计学差异,SAA组的免疫球蛋白浓度(IgM,IgG)低于NSAA组,根据患者感染率进行分析,发现SAA组的感染率明显高于NSAA组。(2)AA组的B淋巴细胞亚群中CD20+B淋巴细胞计数明显低于对照组；CD19+CD38+B淋巴细胞,CD19+CD5+B淋巴细胞计数明显高于对照组；CD20+B淋巴细胞计数在SAA组和NSAA组之间无差异,而SAA组的CD19+CD38+B淋巴细胞,CD19+CD5+B淋巴细胞计数明显高于NSAA组；(3)根据AA患者对IST应答率来比较,发现除CD20+B淋巴细胞外,无效组的CD19+CD38+,CD19+CD5+B淋巴细胞亚群计数均高于有效组。结论：(1)免疫球蛋白的浓度与AA的感染相关；(2)AA患者活化的B淋巴细增多提示AA患者存在B淋巴细胞介导的体液免疫紊乱；B淋巴细胞功能紊乱患者对IST疗效差。 |
| 英文摘要: |
| Objective： To investigate the changes and clinical significance of B-lymphocyte subsets and the immunoglobulin in AA patients. Methods： Use Rate immune scatter turbidimetry to detect the concentration of immunoglobulin (IgG,IgA and IgM)between AA group and control group. Use flow cytometry assay to detect the expression level of CD20+B lymphocyte, activated CD19+CD38+B lymphocyte, B lymphocyte subsetsCD5+CD19+. Results： (1) There is no significant difference in immunoglobulinconcentration between the AA group and the control group. Compared with the AA patients’infection rate, SAA group is higher than that of NSAA group;(2) The expression level of CD20+B lymphocytes in AA group was significantly lower than that of the control group; The expression level of CD19+CD38+, CD19+CD5+B lymphocytes in AA group was significantly higher than that of the control group; And no difference of CD20+B lymphocytes expression level between SAA group and NSAA group,but CD19+CD38+, CD19+CD5+B lymphocytes expression levels in NSAA were lower that of SAA group ;(3) Compared with the AA patients’ respondingto IST, the effective patients have a significantly lower expression level of CD19+CD38+, CD19+CD5+B lymphocytes than that of transfusion dependent patients , the differences were statistically significant. Conclusions：(1) The infection rate of AA may be associated with immunoglobulin;(2) The increased activated B cells and the disorder of humoral immune;The conventional IST treatment was less effective in B lymphocyte dysfunction patients. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
