浙江中西医结合杂志

赖河青,冯红超.雷公藤红素对舌癌中单核/巨噬细胞的影响[J].浙江中西医结合杂志,2016,26(9):

雷公藤红素对舌癌中单核/巨噬细胞的影响

Influencing monocyte/macrophage’s function after tripterine intervented in OSCC Sby ELISA method to detectHeqing Lai₁ Hongchao Feng₂ Yufeng Song₃( 1. Hangzhou Stomatological Hospital 2.Guiyang Stomatological Hospital 3.Guizhou food and drug admini stration ,Guiyang550004,China)

投稿时间：2016-03-21 修订日期：2016-07-22

DOI：

中文关键词: 雷公藤红素单核/巨噬细胞 VEGF VEGF-C 舌鳞状细胞癌

英文关键词:tripterine, mononuclear-macrophage, VEGF , VEGF-C, oral squamous cell carcinoma .

基金项目:贵州省科技厅计划研究项目[黔教高发(2010)305号]

作者	单位	E-mail
赖河青	杭州口腔医院17楼特需科1705诊室	412947049@qq.com
冯红超^*	贵州贵阳市口腔医院	hongchaof@126.com

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中文摘要:

【】：目的：探讨雷公藤红素(Tripterine,Tri)对人单核/巨噬细胞系(THP-1细胞)分泌VEGF、VEGF-C的影响。方法：在酸性微环境中,加入不同浓度的Tri分别与Tca8113细胞、THP-1细胞+ Tca8113细胞、THP-1细胞一起培养24h、48h、72h后,通过ELISA法检测细胞上清液中的血管内皮生长因子-c(VEGF-C)、血管内皮生长因子(VEGF)的含量,以分析雷公藤红素对这两种生长因子的影响。结果： THP-1细胞、Tca8113细胞分泌VEGF、VEGF-C的数量与培养时间、雷公藤红素浓度、pH值呈负相关(P<0.05)。3.结论：随雷公藤红素浓度的增加和时间的延长,雷公藤红素对单核/巨噬细胞分泌VEGF、VEGF-C因子的抑制作用逐渐增强,从而抑制了舌癌的生长和转移。

英文摘要:

Objective：To investigate the effect of tripterine for VEGF and VEGF-C secretion of THP-1 cells. Methods：In vitro microenvironments , different concentrations tripterinewere common cultured with Tca8113 cell, Tca8113 cell+ THP-1 cell, THP-1 cell after 24 hours,48 hours, 72 hours . We collected cell culture supernatants to detected content of VEGF, VEGF-C by Elisa method.Results： the counts of cells and the counts of cytokines of VEGF and VEGF-C all decreased significantly; and presented significantly inactive related to the time, densities of tripterine, and ph.(P＜0.05) Conclusion: In vitro acid oral squamous-cell carcinoma microenvironment. with the increasing of the concentration of tripterine and the elongation of the treated time, the tripterine can inhibits VEGF and VEGF-C secretion of THP-1 cells, which inhibits growth and metastasis of the OSCC .

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