| 任莉莉,王霖玲,王晓稼.苦参碱诱导人乳腺癌Bcap-37细胞发生自噬与mTOR相关性研究[J].浙江中西医结合杂志,2016,26(9): |
| 苦参碱诱导人乳腺癌Bcap-37细胞发生自噬与mTOR相关性研究 |
| A relevant research between the Matrine induced autophagy and mTOR in human breast cancer Bcap-37 cells |
| 投稿时间：2016-03-16 修订日期：2016-07-20 |
| DOI： |
| 中文关键词: 苦参碱，自噬，mTOR，p70S6K，eIF4E |
| 英文关键词:Matrine, autophagy, mTOR,p70S6K, eIF4E |
| 基金项目:浙江省医药卫生科技计划项目(2013KYB04) |
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| 中文摘要: |
| 目的：明确苦参碱是否可通过抑制mTOR活性阻断PI3K/Akt信号通路,从而诱导人乳腺癌Bcap-37细胞自噬发生。 方法：苦参碱(终浓度为0、1、2mg/ml)±氯喹(Chloroquine)(自噬抑制剂)处理乳腺癌Bcap-37细胞24小时,运用免疫印迹(Western-blot)法检测PI3K/Akt信号通路上的自噬相关蛋白mTOR、p-mTOR、p70S6K、p-p70S6、eIF4E的表达。苦参碱(终浓度为0、1、2mg/ml)±BafilomycinA1(自噬抑制剂)/ Rapamcin(自噬诱导剂)处理乳腺癌Bcap-37细胞24小时,Western-blot法检测eIF4E蛋白的表达。 结果：苦参碱剂量依赖性抑制mTOR、p70S6k的磷酸化,同时降低eIF4E蛋白的表达；当苦参碱联合氯喹(CQ)作用于乳腺癌Bcap-37细胞时,则对上述蛋白表达均无明显抑制作用。苦参碱联合BafilomycinA1作用于Bcap-37细胞时,eIF4E蛋白表达同样无明显改变；苦参碱联合Rapamcin时,eIF4E蛋白表达则呈剂量依赖性降低。 结论：苦参碱可通过抑制mTOR活性阻断PI3K/Akt信号通路,从而诱导人乳腺癌Bcap-37细胞自噬发生。 |
| 英文摘要: |
| Objective: To research whether the matrine induce autophagy in human breast cancer Bcap-37 cells by down-regulating PI3K/Akt signal pathway via inhibition of mTOR or not. Methods: Human breast cancer Bcap-37 cells were cultured in vitro and treated by different final concentrations (0、1、2mg/ml) of matrine ± Chloroquine (autophagy inhibitor), then cultured for 24h . Western-blot assay was used to detect the protein expression level of mTOR, p - mTOR, p70S6K, p - p70S6, eIF4E, which were related of PI3K/Akt signaling pathway. The same cells were cultured in vitro and treated by matrine (0、1、2mg/ml) ± BafilomycinA1 (autophagy inhibitor)/Rapamcin (autophagy revulsant) for 24 hours, Western-blot assay was used to detect the level of eIF4E. Results: Western-blot showed that the expression of p-mTOR, p-p70S6k and eIF4E proteins down-regulated after Bcap-37 cells were treated with Matrine at 0, 1, 2mg/ml for 24h in a does dependent manner, but had no obvious changes after Bcap-37 cells were treated with Matrine CQ for the same while. The expression of eIF4E protein also had no obvious change after Bcap-37 cells were treated with Matrine BafilomycinA1 for 24h, however the expression of eIF4E protein down-regulated after the cells were treated with Matrine Rapamcin in a does dependent manner. Conclusions: The molecular mechanism of autophagy induced by the effect of Matrine in human breast cancer Bcap-37 cells via down-regulatd PI3K/Akt/ mTOR signaling Pathway. |
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