| 骆阳阳,黄琦,冯晓红.人参皂苷对波动性高糖大鼠动脉HO-1、γ-GCS表达的影响[J].浙江中西医结合杂志,2016,26(9): |
| 人参皂苷对波动性高糖大鼠动脉HO-1、γ-GCS表达的影响 |
| Ginseng saponin on volatility sugar in rats Arterial about the influence of HO-1 and γ-GCS’s expression |
| 投稿时间：2016-03-03 修订日期：2016-04-08 |
| DOI： |
| 中文关键词: 人参皂苷，波动性高糖大鼠，动脉，HO-1、γ-GCS表达 |
| 英文关键词:ginsenoside, high blood?glucose fluctuation rat , vascular disease,the expression of HO-1 and γ-GCS |
| 基金项目:浙江省中医药科技计划项目（No.2014ZA045）；浙江省医药卫生科技计划项目（No.201464526） |
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| 中文摘要: |
| 目的：观察人参皂苷对波动性高糖大鼠动脉HO-1、γ-GCS表达的影响。方法：将48只雄性SD大鼠随机分成正常对照组（n=8）和糖尿病模型组（n=40）。高脂饲料喂养糖尿病模型组大鼠2周后用小剂量链脲佐菌素(STZ)诱导建立糖尿病大鼠模型，之后随机分为稳定性高糖组（n=8）和波动性高糖组（n=32），波动性高糖组错时注射葡萄糖、胰岛素制备血糖波动大鼠模型。2周后，将波动性高糖组随机分为4组，分别是人参皂苷低剂量组（14mg/ (kg·d)），人参皂苷中剂量组（28mg/ (kg·d)），人参皂苷高剂量组（56mg/ (kg·d)）及波动性高糖空白组，予人参皂苷低中高剂量干预相对应的模型组8周。实验结束后取大鼠动脉组织测定HO-1、γ-GCS的mRNA及蛋白表达。结果：与正常对照组相比，糖尿病模型组HO-1、γ-GCS的mRNA及蛋白表达明显增加（p<0.05）；与稳定性高糖组比较，波动性高糖空白组、人参皂苷给药组（低、中、高）HO-1、γ-GCS的mRNA表达[(19.66±1.20,15.53±0.87),(21.72±2.27,18.85±1.93),(23.82±0.50,21.65±2.17),(25.90±1.13,26.30±1.68)]比（17.30±0.56，12.50±0.97）均明显增加，差异有统计学意义（p<0.05），蛋白表达量[(64.78±0.25,67.21±0.68),(77.34±0.29,83.48±0.43),(82.65±0.29,94.39±0.22),(92.71±0.20,107.73±1.28)]比(63.28±0.29,59.20±0.66)均明显增加，差异有统计学意义（p<0.05)；与波动性高糖空白组相比，人参皂苷给药组（低、中、高）HO-1、γ-GCS的mRNA表达[(21.72±2.27,18.85±1.93),(23.82±0.50,21.65±2.17),(25.90±1.13,26.30±1.68)]比(19.66±1.20,15.53±0.87)均明显增加,差异有统计学意义（p<0.05）,蛋白表达量[(77.34±0.29,83.48±0.43),(82.65±0.29,94.39±0.22),(92.71±0.20,107.73±1.28)]比（64.78±0.25，67.21±0.68)均明显增加，差异有统计学意义（p<0.05）；与人参皂苷低剂量组相比，人参皂苷中剂量组和人参皂苷高剂量组HO-1、γ-GCS的mRNA表达[(23.82±0.50,21.65±2.17),(25.90±1.13,26.30±1.68)]比(21.72±2.27,18.85±1.93)有上升的趋势,差异有统计学意义（p<0.05）,蛋白表达量[(82.65±0.29,94.39±0.22),(92.71±0.20,107.73±1.28)]比(77.34±0.29,83.48±0.43)有上升的趋势,差异有统计学意义（p<0.05）；与人参皂苷中剂量组相比，人参皂苷高剂量组HO-1、γ-GCS的mRNA表达(25.90±1.13,26.30±1.68)比(23.82±0.50,21.65±2.17)也有上升的趋势,差异有统计学意义（p<0.05）,蛋白表达(92.71±0.20,107.73±1.28)比(82.65±0.29,94.39±0.22)也有上升的趋势,差异有统计学意义（p<0.05）。结论：人参皂苷可增加波动性高血糖大鼠HO-1、γ-GCS的mRNA及蛋白表达水平，减轻血管内皮损伤，因此，人参皂苷可能降低波动性高血糖大鼠机体的氧化应激水平，提高抗氧化的能力，对波动性高血糖所导致的动脉病变有一定的保护作用。 |
| 英文摘要: |
| Objective: To observe the effects of ginsenoside on volatility sugar artery in HO-1 and γ-GCS’s expression of diabetic rats. Methods: Forty eight male Sprague?Dawley(SD) rats were randomly divided into normal group(n = 8)and diabetic model group(n = 40).The diabetic rat model was established by injection of a small dose of streptozotoein(STZ)after feeding them with high?fat diet for 2 weeks, and then they were randomly divided into the sustained high glucose diabetic rat model group（n=8）and the high blood?glucose fluctuation rat model group（n=32）.The model of high blood?glucose fluctuation rat were developed by alternative intraperitoneal injection of insulin and glucose. Successfully -established model rats were randomly divided into four groups：low(14mg/(kg·d)), middle(28mg/(kg·d)), high(56mg/(kg·d)) dosage of ginseng treatment group and control group.After 8 weeks, the expression of mRNA and protein about HO-1 and γ-GCS were measured and observed. Results: Compared with the normal control group, levels of HO-1 and γ-GCS in the diabetic model group were significantly increased(p<0.05);compared with the stability of the high glucose group, levels of HO-1 and γ-GCS about mRNA expression in the high blood?glucose fluctuation control group and ginseng treatment groups were significantly increased, [(19.66±1.20,15.53±0.87),(21.72±2.27,18.85±1.93),(23.82±0.50,21.65±2.17),(25.90±1.13,26.30±1.68)than（17.30±0.56，12.50±0.97）],the difference was statistically significant(p<0.05),in the expression of protein,[(64.78±0.25,67.21±0.68),(77.34±0.29,83.48±0.43),(82.65±0.29,94.39±0.22),(92.71±0.20,107.73±1.28)]than(63.28±0.29,59.20±0.66),the difference was statistically significant(p<0.05);compared with thigh blood?glucose fluctuation rat control group, levels of HO-1 and γ-GCS about mRNA expression in ginseng treatment groups were significantly increased,[(21.72±2.27,18.85±1.93),(23.82±0.50,21.65±2.17),(25.90±1.13,26.30±1.68)]than(19.66±1.20,15.53±0.87),the difference was statistically significant(p<0.05), in the expression of protein,[(77.34±0.29,83.48±0.43),(82.65±0.29,94.39±0.22),(92.71±0.20,107.73±1.28)than (64.78±0.25，67.21±0.68)]were significantly increased,the difference was statistically significant(p<0.05);compared with the low dose ginsenoside group , levels of HO-1 and γ-GCS about mRNA expression in the middle dose ginsenoside group and the high dose ginsenoside group were increased,[(23.82±0.50,21.65±2.17),(25.90±1.13,26.30±1.68)]than(21.72±2.27,18.85±1.93),the difference was existed(p<0.05),in the expression of protein,[(82.65±0.29,94.39±0.22),(92.71±0.20,107.73±1.28)]than(77.34±0.29,83.48±0.43)were increased,the difference was existed(p<0.05);compared with the middle dose ginsenoside group , levels of HO-1 and γ-GCS about mRNA expression in the high dose ginsenoside group were increased(25.90±1.13,26.30±1.68)than(23.82±0.50,21.65±2.17),the difference was existed(p<0.05),in the expression of protein,(92.71±0.20,107.73±1.28)than(82.65±0.29,94.39±0.22)were increased,the difference was existed(p<0.05).Conclusion: ginsenoside can increase the expression level of mRNA and protein about HO-1 and γ-GCS,and reduce vascula endothelial damage.Therefore ginsenoside may reduce the oxidative stress level of high blood?glucose fluctuation rats,improve the antioxidant capacity, which could play a critical role in treating vascular disease caused by high blood-glucose fluctuation. |
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