| 过华蕾,邵畅,龚仪棠,陈豪.白藜芦醇对抗氧化型低密度脂蛋白诱导的内皮细胞损伤及其机制[J].浙江中西医结合杂志,2016,26(8): |
| 白藜芦醇对抗氧化型低密度脂蛋白诱导的内皮细胞损伤及其机制 |
| Protective effect of resveratrol on endothelial injury induced by oxidized low density lipoprotein and the involved mechanism |
| 投稿时间：2016-01-27 修订日期：2016-06-02 |
| DOI： |
| 中文关键词: 白藜芦醇 ox-LDL 氧化应激 动脉粥样硬化 |
| 英文关键词:resveratrol ox-LDL oxidative stress atherosclerosis |
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| 中文摘要: |
| 目的 探讨白藜芦醇对氧化型低密度脂蛋白（ox-LDL）诱导的内皮细胞损伤有无保护作用及其机制。方法 体外培养人脐静脉内皮细胞，并随机分为对照组、ox-LDL组、不同浓度（10μmol/L、50μmol/L）白藜芦醇保护组，通过检测细胞活力、超氧化物歧化酶(SOD)水平、丙二醛(MDA)含量来评价内皮细胞损伤程度。用RT-PCR及Western blot法检测单核细胞趋化蛋白1(MCP-1)和细胞间黏附分子1(ICAM-1) RNA和蛋白表达。结果 与对照组比较，ox-LDL组细胞活力（0.33±0.01 vs 0.45±0.01，P<0.05)、SOD水平（12.8±0.31 U/ml vs 15.5±0.15 U/ml，P<0.05）降低，MDA含量（2.10±0.51nmol/mgprot vs 1.05±0.06nmol/mgprot，P<0.05）增加，MCP-1、ICAM-1 RNA（1.56±0.06 vs 0.67±0.09，3.62±0.32 vs 1.35±0.21，P<0.05）和蛋白表达明显升高；与ox-LDL组相比，白藜芦醇（10、50μmol/L）保护组细胞活力（0.38±0.02、0.39±0.02 vs 0.33±0.01，P<0.05）、SOD水平（14.4±0.61 U/ml、14.8±0.57 U/ml vs 12.8±0.31 U/ml，P<0.05）升高，MDA含量（1.41±0.15nmol/mgprot、1.37±0.05nmol/mgprot vs 2.10±0.51nmol/mgprot，P<0.05）减少，MCP-1、ICAM-1 RNA（1.03±0.05、0.92±0.08 vs 1.56±0.06，1.88±0.25、1.67±0.16 vs 3.62±0.32，P<0.05）和蛋白表达明显降低。结论 白藜芦醇对ox-LDL诱导的血管内皮细胞损伤有保护作用。 |
| 英文摘要: |
| Objective To investigate effects of resveratrol on endothelial injury induced by oxidized low density lipoprotein (ox-LDL) and its mechanism. Methods In vitro cultured human umbilical vein endothelial cells were randomly divided into control group, ox-LDL group, protective group added different concentrations (10μmol/L, 50μmol/L) of resveratrol. Cell viability, superoxide dismutase (SOD) level and malondialdehyde (MDA) content were detected to evaluate the damage of endothelial cells. Expression of monocyte chemoattractant protein l (MCP-1) and intercellular adhesion molecule 1(ICAM-1) in RNA and protein level were detected by RT-PCR and Western blot respectively. Results Compared to control group, cell viability (0.33±0.01 vs 0.45±0.01, P<0.05) and SOD level (12.8±0.31 U/ml vs 15.5±0.15 U/ml, P<0.05) decreased while MDA content (2.10±0.51 nmol/mgprot vs 1.05±0.06 nmol/mgprot, P<0.05) increased, expression of MCP-1 and ICAM-1 in RNA (1.56±0.06 vs 0.67±0.09, 3.62±0.32 vs 1.35±0.21, P<0.05) and protein level increased significantly. Compared to ox-LDL group, cell viability（0.38±0.02、0.39±0.02 vs 0.33±0.01, P<0.05） and SOD level（14.4±0.61 U/ml 、14.8±0.57 U/ml vs 12.8±0.31 U/ml, P<0.05）increased while MDA content（1.41±0.15 nmol/mgprot、1.37±0.05 nmol/mgprot vs 2.10±0.51 nmol/mgprot, P<0.05）decreased, expression of MCP-1 and ICAM-1 in RNA（1.03±0.05、0.92±0.08 vs 1.56±0.06, 1.88±0.25、1.67±0.16 vs 3.62±0.32，P<0.05）and protein level decreased significantly in resveratrol (10、50μmol/L) group.
Conclusion: Resveratrol can protect vascular endothelial cells against ox-LDL-induced injury. |
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