| 施琳琳,陈建永,徐虹,章佳颖.大黄素维持肝硬化大鼠高动力循环状态下肠屏障完整性的研究[J].浙江中西医结合杂志,2016,26(5): |
| 大黄素维持肝硬化大鼠高动力循环状态下肠屏障完整性的研究 |
| Effects of emodin on maintaining intestinal barrier in Cirrhotic rats with Portal hypertension |
| 投稿时间：2015-11-17 修订日期：2016-01-20 |
| DOI： |
| 中文关键词: 肝硬化 门脉高压 肠屏障 大黄素 |
| 英文关键词:Portal Hypertension Intestinal Barrier Emodin |
| 基金项目:浙江省医药卫生科技项目(2011RCB029) |
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| 中文摘要: |
| 目的：观察大黄素对肝硬化大鼠高动力循环状态下肠屏障完整性的维持作用。方法：SD雄性大鼠25只，随机分为对照组5只、肝硬化模型组10只、大黄素干预组10只，模型组与干预组予500ml/L四氯化碳(3mL/100g)皮下注射8周复制肝硬化大鼠模型，对照组大鼠给予生理盐水(3 ml/100g)皮下注射。干预组制模第15天起予大黄素（5mg/mL，5mL/kg）灌胃，对照组和模型组每日给予相当体积的生理盐水灌胃，均1天1次。 8周后处死大鼠，行墨汁推进试验测定肠道传输功能，测定门脉压力，血清生化指标测定，取末端回肠组织及肝脏组织观察组织病理学改变，肠黏膜TUNEL凋亡检测。结果：（1）大鼠小肠黏膜损伤评分：模型组大鼠明显高于对照组（P值＜0.05），干预组较模型组黏膜损伤减轻。（2）门脉压力:模型组（13.73±1.81）较对照组（5.64±0.88）显著升高， P值＜0.05，大鼠明显处于高循环状态；干预组门脉压力（10.25±1.47）较模型组明显降低，P值＜0.05。（3）血清生化指标（ALT、AST、TB、ALP）水平：模型组(594.22±317.82,1008.33±778.70,6.00±5.29,
802.78±396.94)较对照组(60.20±21.30,149.80±43.03,1.00±0.44,196.20±31.29)显著升高， P值＜0.05，干预组(292.20±140.12,350.40±173.35,18.89±1.02,2.49±1.10,552.20±303.37)较模型组明显降低，P值＜0.05；ALB模型组(16.29±1.26)较对照组(23.42±1.56)明显下降， P值＜0.05，干预组(2.49±1.10)较模型组明显上升，P值＜0.05（4）肠道黑染百分比: 模型组（68.05±2.09）较对照组（81．68±3.15）明显减少， P值＜0.05， 干预组(74.50±4.28)肠道黑染百分比较模型组明显延长，P值＜0.05；（5）肠黏膜凋亡小体：模型组明显多于对照组，而干预组大黄素干预后的小肠上皮细胞凋亡较模型组明显下降。结论：应用大黄素干预大鼠肝硬化进程后，大黄素组大鼠肝脏胶原纤维与网状纤维沉积较少，假小叶少见。大鼠的门脉压力有明显的下降，大黄素组大鼠肝功能明显好转，光镜下小肠黏膜损伤评分显著降低，肠动力明显好转，凋亡减少，大黄素可保护肝硬化门脉高压大鼠的肠屏障功能。 |
| 英文摘要: |
| Objective :To investigate the effects of emodin on intestinal mncosal barrier in cirrhotic rat with Portal hypertension. Methods:25 male SD rats were randomly divided into three groups: control group(n=5), model group(n=10), emodin group(n=10).The model was induced by the subcutaneous injection of 500ml/l CCL4((3ml/100g)(twice a week for 8 weeks), （5mg/ml，5ml/kg）emodin was administrated intragastrically in emodin group from the fifteenth day,once a day. while the control group received normal saline instead. days．The same volume saline was administra—ted intragastrically in control and model group.The changes of Ink propelling test, Portal pressure, Histology, apoptosis body in those rats were observed. Results: The Degree of intestinal mucosal injury, Portal pressure,apoptosis body and Hepatic function in model group were higher than control group, while Degree of intestinal mucosal injury, Portal pressure,apoptosis body and Hepatic function in emodin group were lower than model group; The percentage of dyed intestinal black in emodin group was higher than model group. Conclusion Emodin can alleviate intestinal pathological damages，reduce portal pressure, improve intestinal motility, emodin could protect intestinal mucosal barrier in cirrhotic rats with Portal hypertension． |
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