| 陈素珍,楼黎明,胡丹丹,黄立搜,王世强,徐一凯,章潜.“肺肠同治”法对脓毒症大鼠肺基质金属蛋白酶及其抑制物表达的影响[J].浙江中西医结合杂志,2016,26(8): |
| “肺肠同治”法对脓毒症大鼠肺基质金属蛋白酶及其抑制物表达的影响 |
| Keywordsrats;sepsis; Treat lung and colon together; MMP-9；TIMP-1 |
| 投稿时间：2015-11-09 修订日期：2016-05-10 |
| DOI： |
| 中文关键词: 大鼠 脓毒症 肺肠同治 MMP-9 TIMP-1 |
| 英文关键词:rats sepsis Treat lung and colon together MMP-9 TIMP-1 |
| 基金项目:浙江省中管局课题（2012ZB069）;浙江中医药大学课题(2013ZR03) |
|
| 摘要点击次数: 820 |
| 全文下载次数: 1 |
| 中文摘要: |
| 根据“肺合大肠”理论拟定治肺、治肠、肺肠同治方药，观察不同治法对脓毒症急性肺损伤大鼠的肺组织匀浆中MMP-９、TIMP-1及MMP-９/TIMP-1比值的变化，探讨“肺合大肠”理论治疗脓毒症肺损伤的作用机制。方法 将清洁级SD雄性大鼠50只随机分为5组：假手术组、模型组、治肺组、治肠组、肺肠同治组，每组10只。以盲肠结扎穿孔（CLP）法制备脓毒症大鼠模型，观察CLP术后24h大鼠肺组织匀浆中MMP-９、TIMP-1及MMP-９/TIMP-1比值、肺湿/干重比（W/D）的情况。结果 与假手术组比较，模型组治肺组、治肠组、肺肠同治组大鼠肺湿/干重比均明显增大（P＜0.01）；与模型组比较，治肺组、治肠组、肺肠同治组的大鼠肺湿/干重比均显著减小（P＜0.01或P＜0.05）。与假手术组比较，模型组、治肺组、治肠组、肺肠同治组的大鼠MMP-９、TIMP-1 显著增高（P＜0.01或P＜0.05），而TIMP-1/MMP-９比值显著下降（P＜0.01或P＜0.05）。与模型组比较，治肺组、治肠组、肺肠同治组大鼠MMP-９[(12.56±0.76),(12.50±0.98),(10.50±0.71)，比 (14.80±0.59)低，P＜0.05]、TIMP-1[(14.10±1.12),(14.30±1.30),(10.90±0.53)， 比(17.70±1.06)低，P＜0.05]显著下降（P＜0.01或P＜0.05），而TIMP-1/MMP-９比值[(0.91±0.04),( 0.90±0.04),( 0.98±0.03), 比(0.84±0.03)升高，P＜0.05]显著升高（P＜0.01或P＜0.05）。结论 “治肺”法、“治肠”法、“肺肠同治”法均可以下调MMP-9基因表达，上调TIMP-1基因表达，从而减轻脓毒症的肺损伤，对肺脏有一定的保护作用，其中以肺肠同治法效果最好。运用“肺合大肠”理论治疗脓毒症肺损伤有一定的作用机制。 |
| 英文摘要: |
| Objective According to the "Treat lung and colon together” theory, we developed prescriptions for treatment of lung, colon and both, respectively, observed the effects of these prescriptions and the serum and lung level of MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio in acute lung injury in rats, and explore the underlying mechanisms of Methods SD 50 male rats were randomly divided into five groups with 10 in each group: sham operation group, model group, lung treatment group, colon treatment group, lung and colon treatment. Cecal ligation and puncture (CLP) model was used to generate sepsis in rats, MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio in serum and lung tissue, arterial blood gas (ABG) and lung wet/dry weight ratio (W/D) were observed 24h after CLP. Results Compared with the model group, ABG and W/D ratios were significantly improved lung, colon or lung and colon treatment groups, but more prominent in lung and colon treatment group (P <0.01 or P <0.05). Compared with the model group, MMP-9 and TIMP-1 in lung and serum were significantly decreased, and TIMP-1/MMP-９ratios significant improved in treatment groups (P <0.01 or P <0.05). All these changes are more pronounced in long and colon treatment group than lung, or colon treatment groupds. Conclusion ABG and W/D ration were significant improved in treatment groups. Reduction of MMP-9 and TIMP-1 expression in all treatment groups, which is more pronounced in lung and colon treatment group, could be the underlying mechanism. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
