| 邵笑,祝骥,赵峻峰,许颖龄,张颖颖,杨建清,卢德赵.丹参酮ⅡA对肺纤维化细胞TGF-β1/Smads信号通路的影响[J].浙江中西医结合杂志,2016,26(5): |
| 丹参酮ⅡA对肺纤维化细胞TGF-β1/Smads信号通路的影响 |
| Effect of Tanshinone ⅡA on TGF-β1/Smads Signaling Pathway in Pulmonary Fibrosis Cells |
| 投稿时间：2015-08-20 修订日期：2015-11-19 |
| DOI： |
| 中文关键词: 丹参酮ⅡA 肺纤维化 转化生长因子β |
| 英文关键词:Pulmonary fibrosis TGF-β1 Smad Tanshinone ⅡA |
| 基金项目:国家自然科学(81102841,81403133)；浙江省自然科学(LY13H270005,LQ14H280004)；中国博士后科学基金(2013M541800) |
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| 中文摘要: |
| 目的：观察丹参酮ⅡA对TGF-β1诱导大鼠肺成纤维细胞TGF-β1/Smads信号通路的影响,阐明丹参酮ⅡA干预肺纤维化的机制。方法：体外培养大鼠肺成纤维细胞,经TGF-β1、TGF-β1/丹参酮ⅡA处理24h,用ELISA法测定细胞中TGF-β1受体表达情况,荧光定量PCR法及免疫印迹法检测Smad2、Smad7表达情况。 结果：TGF-β1处理后TGF-β1受体和Smad2表达量增加,Smad7表达量降低,丹参酮ⅡA处理后TGF-β1受体和Smad2的表达量均有下降趋势,Smad7表达量升高。结论：丹参酮ⅡA可以抑制TGF-β1诱导的大鼠肺成纤维细胞增殖和Smad2及TGF-β1受体表达,增强抑制性信号蛋白Smad7的表达,从而发挥抑制肺纤维化的作用。 |
| 英文摘要: |
| Objective: To investigate the protection effect of tanshinone ⅡA on TGF-β1/Smads pathway in pulmonary fibrosis cells. Methods: HLF-1 cells were cultivated in vitro and treated with the optimal concentration of TGF-β1, TGF-β1/tanshinoneⅡA respectively for 24 h. The method of Elisa was used to measure the expression of TGF-β1 receptors. The expression of Smad 2 and Smad 7 was determined with the method of fluorogenic quantitative PCR and western blotting. Results: TGF-β1 could cause serious damage to the HLF-1 cells which can increase the expression of TGF-β1 receptors and Smad 2, and decrease the expression of Smad 7. When the cells were treated with Tanshinone ⅡA, TGF-β1 receptors and Smad 2 were expressed with a downward trend while expression of Smad 7 was increased. Conclusion: TGF-β1 can promote pulmonary fibrosis through upregulating the expression of TGF-β1 receptors and Smad 2 and downregulating the expression of Smad 7. Tanshinone ⅡA can regulate the TGF-β1/Smads signaling pathway, which plays a preventive role in pulmonary fibrosis. |
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