| 徐芳芳,马伟明,高志斌,康年松.中药肠愈宁对溃疡性结肠炎癌变的干预作用[J].浙江中西医结合杂志,2015,25(10): |
| 中药肠愈宁对溃疡性结肠炎癌变的干预作用 |
| Intervention Effect of Traditional Chinese Medicine (TCM) Chang Yu Ning on Ulcerative Colitis Canceration |
| 投稿时间：2015-04-17 修订日期：2015-05-23 |
| DOI： |
| 中文关键词: 溃疡性结肠炎,癌变,中药,氧化偶氮甲烷,葡聚糖硫酸钠 |
| 英文关键词:ulcerative colitis, cancaration, Chinese medicine, AOM, DSS |
| 基金项目: |
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| 摘要点击次数: 795 |
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| 中文摘要: |
| [目的] 观察自拟中药方肠愈宁预防、抑制溃疡性结肠炎癌变的作用。
[方法] 将60只健康SD大鼠随机分为六组,采用氧化偶氮甲烷/葡聚糖硫酸钠（Azoxymethane, AOM / Dextran Sodium Sulfate, DSS）化学诱导方法构建溃疡性结肠炎癌变大鼠模型：模型组、中药低剂量组（低剂量组）、中药中剂量组（中剂量组）、中药高剂量（高剂量组）和美沙拉嗪组先腹腔注射AOM 10mg/kg，浓度为2mg/ml，1周后开始自由饮用3% DSS溶液，饮用14天，此为一个循环，连续3个循环。造模开始第一天开始给药，每天一次，连续给药9周。空白组不作任何处理。实验过程中观察大鼠一般情况、大便性状、体重变化等。实验结束，解剖观察大鼠结肠大体形态，做HE染色，观察结肠组织病理形态学变化。
[结果] （1）模型组大体形态：符合溃疡性结肠炎及癌变表现特点。（2）模型组的病理结果：结肠粘膜轻度异型增生20.0%（2/10），中度异型增生30.0%（3/10），重度异型增生10.0%（1/10）。（3）各组DAI评分比较：低剂量组、中剂量组、美沙拉嗪组和模型组比较有显著性差异，P<0.05。（4）各组结肠大体形态评分比较：模型组、低剂量组、中剂量组、高剂量组、美沙拉嗪组与空白组比较差异显著，P<0.05。低剂量组、高剂量组与美沙拉嗪组比较差异显著，P<0.05。（5）各组结肠组织学损伤评分比较结果：低剂量组与模型组比较差异显著，P<0.05。
[结论] 自拟中药方肠愈宁能明显减轻溃疡性结肠炎癌变大鼠模型的临床症状，改善组织病理类型，减少溃疡性结肠炎癌变。 |
| 英文摘要: |
| Objective To observe the prevention and control effect of ChangYuning on ulcerative colitis canceration and the expression of K-ras, DCC,DPC4 and APC under different forms of colonic mucosa in rats, aiming to provide theory basis for early identification and prevention of ulcerative colitis canceration through immune indexes.
Methods 60 healthy SD rats were randomized into six groups: the blank model group, the mesalazine group, the Chinese medicine of low dose group (LD), the middle does group (MD), the high does group (HD) and the blank group. Azoxymethane(AOM) and dextran sodium sulfate(DSS) were used in this study to establish rat models of ulcerative colitis canceration. Specific methods: First, rats in blank model group, LD, MD, HD and mesalazine group got AOM 10mg/kg, concentration of 2mg/ml by intraperitoneal injection. A week later, rats in these groups started to drink 3% DSS for 14 days. This was experimented as one circle, totally 3 circles continuously while rats in blank group received no conduct, eating and drinking freely. Rats in normal group and blank model group were given distilled water and the rests were given the drugs by gavage once a day on the first day of modeling, 9 weeks continuously. We Observed the general situation, stool, daily weight and so on during the experiment. After the experiment, dissected rats to observe the morphology of colon, made HE staining to observe colon-histopathological changing.
Results (1) General morphology of the model group: it conforms to the manifestations of ulcerative colitis and cancaration; (2) Pathological results of the model group: there are 20.0% (2/10) colon mucosa mild dysplasia, 30.0%(3/10)moderate dysplasia and 10.0% (1/10) severe dysplasia; (3) Results of DAI comparison: there’s significant difference between model group and mesalazine group, LD& MD, P < 0.05, while mesalazine group, LD, MD and HD have no significant difference, P > 0.05, which suggests both Chinese medicine and western medicine mesalazine can improve DAI of UC rats and alleviate the clinical symptoms, but Chinese medicine and western medicine have no obvious difference in improving DAI. (4) Results of morphology score comparison: there’s significant difference between model group and mesalazine group, LD, MD, HD & blank group, P < 0.05，same as LD and HD, mesalazine group, P < 0.05, while no significant difference between MD and mesalazine group, LD & HD, P > 0.05, which suggests Chinese medicine and western medicine both have an effect on improving morphology of colons, but the right dose of Chinese medicine seems to be more efficacious. (5) Results of colon-histopathological score comparison: there’s significant difference between model group and LD, P < 0.05, suggesting that Chinese medicine can significantly improve endoscopic manifestation of the colon mucosal. There’s no obvious difference between model group and mesalazine group, MD & HD, P > 0.05, and mesalazine group, LD, MD and HD also have no obvious difference, P > 0.05, both of which suggest Chinese medicine and western medicine have no significant difference in alleviating damage of colon mucosal .
Conclusion Chinese medicine Chang Yuning can obviously relieve the clinical symptoms of the rats with ulcerative colitis canceration, improve histologic type and reduce the risk of ulcerative colitis canceration. |
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