| 夏佳佳,王岚,章燕珍.高浓度葡萄糖对牙周膜干细胞成骨分化能力的影响[J].浙江中西医结合杂志,2015,25(7): |
| 高浓度葡萄糖对牙周膜干细胞成骨分化能力的影响 |
| Effects of highglucose on proliferation and osteogenic differentiation of periodontal ligament stem cells in vitro |
| 投稿时间：2015-03-06 修订日期：2015-03-30 |
| DOI： |
| 中文关键词: 葡萄糖 牙周膜干细胞 成骨诱导 |
| 英文关键词:glucose periodontal ligament stem cells osteogenic differentiation |
| 基金项目: |
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| 摘要点击次数: 871 |
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| 中文摘要: |
| 目的 研究高葡萄糖浓度刺激下牙周膜干细胞(PDLSCs)的增殖能力和成骨分化能力。方法 组织块法体外培养非糖尿病患者PDLSCs,分别用0mg/L(C组)、1100mg/L(L组)、4500 mg/L(H组)浓度葡萄糖刺激,MTT法检测PDLSCs增殖能力,矿化诱导后茜素红染色观察矿化结节形成,实时定量聚合酶链反应(RT-PCR)检测Runt相关转录因子2(Runx-2)、碱性磷酸酶(alkaline phosphatase,ALP)和I型胶原(collagen type I,Col-1)成骨相关基因表达。结果 MTT法检测显示H组OD值较L组和C组低(P＜0.05)；21天成骨诱导后,H组矿化结节面积较L组和C组少(P＜0.05)；成骨诱导期间,H组ALP、Runx2和Col-1诱导前后相对倍增数较L组和C组低(P＜0.05)。结论 高浓度葡萄糖抑制PDLSCs增殖能力和成骨分化能力。 |
| 英文摘要: |
| Objective：To isolate the periodontal ligament stem cells(PDLSCs) and studied the effect of high glucose concentration levels on the cellular activity and osteogenic differentiation of PDLSCs from non-diabetic patients in vitro.Methods: PDLSCs were cultured for 14 days in a low glucose medium (1100 mg/L of glucose) or in a high glucose medium (4500 mg/L of glucose),respectively, a 3-(4,5-dimethylithiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay for cellular viability was performed, the osteogenic differentiation capacity of PDLSCs was evaluated by alizarin red staining and real-time quantitative reverse transcription polymerase chain reaction(real time RT-PCR).Results:High glucose signi?cantly inhibited the proliferation of PDLSCs and reduced the optic density(OD) of the MTT, the difference was a significant(P﹤0.05), Concerning the mineralized nodule formation, the percentage of the calci?ed area and the expression of osteogenic genes (ALP, Runx-2, Col-1) to the total culture dish of PDLSCs in high glucose level were significant lower than that in the low glucose medium and control medium(P﹤0.05). Conclusion: High glucose inhibits the proliferation and differentiation of PDLSCs. |
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